Toxicity evaluation of 6-mercaptopurine-Chitosan nanoparticles in rats

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Autor(es): dc.contributorThe Pennsylvania State University-
Autor(es): dc.contributorVeterinary College-
Autor(es): dc.contributorUniversity of South Florida-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorInnovative Nano and Micro Technologies Private Limited-
Autor(es): dc.creatorGovindappa, Prem Kumar-
Autor(es): dc.creatorJoladarashi, Darukeshwara-
Autor(es): dc.creatorHallur, Raghavendra Lakshmana Shetty [UNESP]-
Autor(es): dc.creatorSanganal, Jagadeesh S.-
Autor(es): dc.creatorPhani, Ayyalasomayajula Ratna-
Data de aceite: dc.date.accessioned2022-02-22T00:28:38Z-
Data de disponibilização: dc.date.available2022-02-22T00:28:38Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2019-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.jsps.2019.11.018-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/199875-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/199875-
Descrição: dc.descriptionBackground: The 6-mercaptopurine (6-MP) is an effective immunosuppressant and anti-cancer drug. However, the usage of 6-MP is limited due to its well-known side effects, such as myelotoxicity and hepato-renal toxicity. To curtail the potential toxic effects, we have used chitosan as a natural biodegradable and biocompatible polysaccharide to synthesize 6-Mercaptopurine-Chitosan Nanoparticles (6-MP-CNPs). Methods: The 6-MP-CNPssize, morphology, physicochemical interactions, and thermal stability were characterized using Dynamic Light Scattering (DLS), Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR), and Differential Scanning Calorimetry (DSC), respectively. The loading efficiency of the 6-MP in CNPs was estimated using LCMS/MS. Then, the 6-MP-CNPs were subjected to in vivo acute and sub-acute oral toxicity evaluations. Results: The DLS and SEM analysis respectively indicated size (70.0 nm to 400.0 nm), polydispersity index (0.462), and zeta potential (54.9 mV) with improved morphology of 6-MP-CNPs. The FTIR and DSC results showed the efficient interactive and stable nature of the 6-MP-CNPs, which sustained the drug-delivery process. The loading efficiency of 6-MP-CNPs was found to be 25.23%. The chitosan improved the lethal dose (LD50 cut off) of 6-MP-CNPs (1000 mg/kg b.w) against 6-MP (500 mg/kg b.w) and also significantly (p ≤ 0.05) reduces the toxic adverse effect (28-day repeated oral dose) on hemato-biochemical and hepato-renal histological profiles. Conclusion: The findings suggest that chitosan, as a prime drug-delivery carrier, significantly alleviates the acute and sub-acute toxic effects of 6-MP.-
Descrição: dc.descriptionH2020 LEIT Nanotechnologies-
Descrição: dc.descriptionDepartment of Orthopaedics and Rehabilitation College of Medicine The Pennsylvania State University-
Descrição: dc.descriptionDepartment of Veterinary Pharmacology and Toxicology Veterinary College, Hebbal-
Descrição: dc.descriptionDepartment of Molecular Pharmacology and Physiology Morsani College of Medicine University of South Florida-
Descrição: dc.descriptionDepartment of Gynecology and Obstetrics Botucatu Medical School (FMB) São Paulo State University (UNESP)-
Descrição: dc.descriptionInnovative Nano and Micro Technologies Private Limited, Mysore Road-
Descrição: dc.descriptionDepartment of Gynecology and Obstetrics Botucatu Medical School (FMB) São Paulo State University (UNESP)-
Formato: dc.format147-154-
Idioma: dc.languageen-
Relação: dc.relationSaudi Pharmaceutical Journal-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subject6-Mercaptopurine-
Palavras-chave: dc.subjectAnti-cancer-
Palavras-chave: dc.subjectChitosan-
Palavras-chave: dc.subjectNanoparticle-
Palavras-chave: dc.subjectToxicity-
Título: dc.titleToxicity evaluation of 6-mercaptopurine-Chitosan nanoparticles in rats-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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