Role of neuroendocrine modulation and biochemistry in the sepsis in Piaractus mesopotamicus

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUFOPA-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorFUNEPE-
Autor(es): dc.contributorUniversidade Federal de Uberlândia (UFU)-
Autor(es): dc.contributorIMARPE-
Autor(es): dc.creatorClaudiano, Gustavo S. [UNESP]-
Autor(es): dc.creatorAndrade, Sónia C.S.-
Autor(es): dc.creatorSouza, Elaine C.-
Autor(es): dc.creatorYunis-Aguinaga, Jefferson [UNESP]-
Autor(es): dc.creatorCoutinho, Luiz L.-
Autor(es): dc.creatorMoreira, Débora K.T.-
Autor(es): dc.creatorGonçalves, Felipe C.-
Autor(es): dc.creatorMundim, Antonio V.-
Autor(es): dc.creatorMarzocchi-Machado, Cleni M.-
Autor(es): dc.creatorde Moraes, Flávio R. [UNESP]-
Autor(es): dc.creatorMoraes, Julieta R.E. [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:28:33Z-
Data de disponibilização: dc.date.available2022-02-22T00:28:33Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-03-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.ygcen.2019.113338-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/199846-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/199846-
Descrição: dc.descriptionSepsis is a systemic process with multifactorial pathophysiology that affects most animal species. It is responsible for high rates of morbidity and mortality. This work aimed to study the biochemical and neuroendocrine changes of the sepsis process in Piaractus mesopotamicus after Aeromonas hydrophila inoculation analyzing changes in blood leukocyte and differences in neuroendocrine-biochemical modulation using RNA-seq. Fish showed hypercortisolemia, inhibition of glucose absorption, followed by hypocortisolemia and then hyperglycemia. Thyroid hormones (T3 and T4) showed immediate decrease in serum and T4 increased 6 h post-inoculation (HPI). Sepsis-induced hormonal alterations triggered changes in the metabolic pathways increasing protein and lipid catabolism, use of transient anaerobic glycolysis and liver injury. A reference transcriptome was constructed based on blood leukocytes from P. mesopotamicus. The assembly resulted in total 266,272 contigs with a N50 of 2786 bp. There was a reorganization of plasma membrane of leukocytes at the beginning of the septic process with increased expression of neuroendocrine receptors and with continuous flow of neurotransmitters, hormones and solutes with compensatory regulation at 6 HPI. Three and nine HPI seemed to be critical, the expression of a number of transcription factors was increased, including the modulatory DEGs related to glucocorticoid and thyroid hormones induced and suppressed (FDR < 0.05). Neuroendocrine modulation can regulate leukocytes and biochemical parameters of peripheral blood, being important sources for the study of the pathophysiology of sepsis. These finding highlights the importance of further studies focusing on biochemical-neuroendocrine changes in blood leukocytes and systemic sepsis.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionDepartment of Veterinarian Pathology Faculty of Agrarian and Veterinarian Sciences São Paulo State University, Unesp, Jaboticabal-
Descrição: dc.descriptionInstitute of Biodiversity and Forests Federal University of Western Pará UFOPA-
Descrição: dc.descriptionDepartment of Genetics and Evolutionary Biology Institute of Biosciences São Paulo University USP-
Descrição: dc.descriptionEducational Foundation of Penápolis FUNEPE, Penápolis-
Descrição: dc.descriptionAquaculture Center of UNESP, Jaboticabal-
Descrição: dc.descriptionDepartment of Animal Science São Paulo University USP ESALQ-
Descrição: dc.descriptionClinical Analysis Laboratory Veterinary Hospital Federal University of Uberlândia UFU, Uberlândia-
Descrição: dc.descriptionDepartment of Clinical Analyses Toxicology and Food Sciences School of Pharmaceutical Sciences of Ribeirão Preto University of São Paulo USP-
Descrição: dc.descriptionInstituto del Mar del Perú IMARPE-
Descrição: dc.descriptionDepartment of Veterinarian Pathology Faculty of Agrarian and Veterinarian Sciences São Paulo State University, Unesp, Jaboticabal-
Descrição: dc.descriptionAquaculture Center of UNESP, Jaboticabal-
Descrição: dc.descriptionFAPESP: 2011/20280-2-
Descrição: dc.descriptionFAPESP: 2015/12143-6-
Descrição: dc.descriptionCNPq: 441054/2014-5-
Idioma: dc.languageen-
Relação: dc.relationGeneral and Comparative Endocrinology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAeromonas hydrophila-
Palavras-chave: dc.subjectHormones-
Palavras-chave: dc.subjectLeukocytes-
Palavras-chave: dc.subjectPacu-
Palavras-chave: dc.subjectRNA-seq-
Título: dc.titleRole of neuroendocrine modulation and biochemistry in the sepsis in Piaractus mesopotamicus-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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