P-MAPA and IL-12 Differentially Regulate Proteins Associated with Ovarian Cancer Progression: A Proteomic Study

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Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorFarmabrasilis RandD Division-
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.creatorJúnior, Luiz Antonio Lupi-
Autor(es): dc.creatorCucielo, Maira Smaniotto-
Autor(es): dc.creatorDomeniconi, Raquel Fantin-
Autor(es): dc.creatorDos Santos, Lucilene Delazari [UNESP]-
Autor(es): dc.creatorSilveira, Henrique Spaulonci-
Autor(es): dc.creatorDa Silva Nunes, Iseu-
Autor(es): dc.creatorMartinez, Marcelo-
Autor(es): dc.creatorMartinez, Francisco Eduardo-
Autor(es): dc.creatorFávaro, Wagner José-
Autor(es): dc.creatorChuffa, Luiz Gustavo De Almeida-
Data de aceite: dc.date.accessioned2022-02-22T00:28:30Z-
Data de disponibilização: dc.date.available2022-02-22T00:28:30Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2019-12-23-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1021/acsomega.9b02512-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/199836-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/199836-
Descrição: dc.descriptionTo investigate the potential role of immunotherapies in the cellular and molecular mechanisms associated with ovarian cancer (OC), we applied a comparative proteomic toll using protein identification combined with mass spectrometry. Herein, the effects of the protein aggregate magnesium-ammonium phospholinoleate-palmitoleate anhydride, known as P-MAPA, and the human recombinant interleukin-12 (hrIL-12) were tested alone or in combination in human SKOV-3 cells. The doses and period were defined based on a previous study, which showed that 25 μg/mL P-MAPA and 1 ng/mL IL-12 are sufficient to reduce cell metabolism after 48 h. Indeed, among 2,881 proteins modulated by the treatments, 532 of them were strictly concordant and common. P-MAPA therapy upregulated proteins involved in tight junction, focal adhesion, ribosome constitution, GTP hydrolysis, semaphorin interactions, and expression of SLIT and ROBO, whereas it downregulated ERBB4 signaling, toll-like receptor signaling, regulation of NOTCH 4, and the ubiquitin proteasome pathway. In addition, IL-12 therapy led to upregulation of leukocyte migration, tight junction, and cell signaling, while cell communication, cell metabolism, and Wnt signaling were significantly downregulated in OC cells. A clear majority of proteins that were overexpressed by the combination of P-MAPA with IL-12 are involved in tight junction, focal adhesion, DNA methylation, metabolism of RNA, and ribosomal function; only a small number of downregulated proteins were involved in cell signaling, energy and mitochondrial processes, cell oxidation and senescence, and Wnt signaling. These findings suggest that P-MAPA and IL-12 efficiently regulated important proteins associated with OC progression; these altered proteins may represent potential targets for OC treatment in addition to its immunoadjuvant effects.-
Descrição: dc.descriptionDepartment of Anatomy Institute of Biosciences-
Descrição: dc.descriptionCenter for the Study of Venoms and Venomous Animals (CEVAP) UNESP - Universidade Estadual Paulista-
Descrição: dc.descriptionFarmabrasilis RandD Division-
Descrição: dc.descriptionDepartment of Structural and Functional Biology UNICAMP - University of Campinas-
Descrição: dc.descriptionDepartment of Morphology and Pathology Federal University of São Carlos-
Descrição: dc.descriptionCenter for the Study of Venoms and Venomous Animals (CEVAP) UNESP - Universidade Estadual Paulista-
Formato: dc.format21761-21777-
Idioma: dc.languageen-
Relação: dc.relationACS Omega-
???dc.source???: dc.sourceScopus-
Título: dc.titleP-MAPA and IL-12 Differentially Regulate Proteins Associated with Ovarian Cancer Progression: A Proteomic Study-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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