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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.contributor | Farmabrasilis RandD Division | - |
Autor(es): dc.contributor | Universidade Estadual de Campinas (UNICAMP) | - |
Autor(es): dc.contributor | Universidade Federal de São Carlos (UFSCar) | - |
Autor(es): dc.creator | Júnior, Luiz Antonio Lupi | - |
Autor(es): dc.creator | Cucielo, Maira Smaniotto | - |
Autor(es): dc.creator | Domeniconi, Raquel Fantin | - |
Autor(es): dc.creator | Dos Santos, Lucilene Delazari [UNESP] | - |
Autor(es): dc.creator | Silveira, Henrique Spaulonci | - |
Autor(es): dc.creator | Da Silva Nunes, Iseu | - |
Autor(es): dc.creator | Martinez, Marcelo | - |
Autor(es): dc.creator | Martinez, Francisco Eduardo | - |
Autor(es): dc.creator | Fávaro, Wagner José | - |
Autor(es): dc.creator | Chuffa, Luiz Gustavo De Almeida | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:28:30Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:28:30Z | - |
Data de envio: dc.date.issued | 2020-12-11 | - |
Data de envio: dc.date.issued | 2020-12-11 | - |
Data de envio: dc.date.issued | 2019-12-23 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1021/acsomega.9b02512 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/199836 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/199836 | - |
Descrição: dc.description | To investigate the potential role of immunotherapies in the cellular and molecular mechanisms associated with ovarian cancer (OC), we applied a comparative proteomic toll using protein identification combined with mass spectrometry. Herein, the effects of the protein aggregate magnesium-ammonium phospholinoleate-palmitoleate anhydride, known as P-MAPA, and the human recombinant interleukin-12 (hrIL-12) were tested alone or in combination in human SKOV-3 cells. The doses and period were defined based on a previous study, which showed that 25 μg/mL P-MAPA and 1 ng/mL IL-12 are sufficient to reduce cell metabolism after 48 h. Indeed, among 2,881 proteins modulated by the treatments, 532 of them were strictly concordant and common. P-MAPA therapy upregulated proteins involved in tight junction, focal adhesion, ribosome constitution, GTP hydrolysis, semaphorin interactions, and expression of SLIT and ROBO, whereas it downregulated ERBB4 signaling, toll-like receptor signaling, regulation of NOTCH 4, and the ubiquitin proteasome pathway. In addition, IL-12 therapy led to upregulation of leukocyte migration, tight junction, and cell signaling, while cell communication, cell metabolism, and Wnt signaling were significantly downregulated in OC cells. A clear majority of proteins that were overexpressed by the combination of P-MAPA with IL-12 are involved in tight junction, focal adhesion, DNA methylation, metabolism of RNA, and ribosomal function; only a small number of downregulated proteins were involved in cell signaling, energy and mitochondrial processes, cell oxidation and senescence, and Wnt signaling. These findings suggest that P-MAPA and IL-12 efficiently regulated important proteins associated with OC progression; these altered proteins may represent potential targets for OC treatment in addition to its immunoadjuvant effects. | - |
Descrição: dc.description | Department of Anatomy Institute of Biosciences | - |
Descrição: dc.description | Center for the Study of Venoms and Venomous Animals (CEVAP) UNESP - Universidade Estadual Paulista | - |
Descrição: dc.description | Farmabrasilis RandD Division | - |
Descrição: dc.description | Department of Structural and Functional Biology UNICAMP - University of Campinas | - |
Descrição: dc.description | Department of Morphology and Pathology Federal University of São Carlos | - |
Descrição: dc.description | Center for the Study of Venoms and Venomous Animals (CEVAP) UNESP - Universidade Estadual Paulista | - |
Formato: dc.format | 21761-21777 | - |
Idioma: dc.language | en | - |
Relação: dc.relation | ACS Omega | - |
???dc.source???: dc.source | Scopus | - |
Título: dc.title | P-MAPA and IL-12 Differentially Regulate Proteins Associated with Ovarian Cancer Progression: A Proteomic Study | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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