Anabolism, catabolism and proteomic analysis in the slow-twitch muscle of pacu (Piaractus mesopotamicus) submitted to prolonged fasting followed by refeeding

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorGabriel Kuniyoshi, Maria Laura [UNESP]-
Autor(es): dc.creatorNunes da Silva-Gomes, Rafaela [UNESP]-
Autor(es): dc.creatorThomazini Zanella, Bruna Tereza [UNESP]-
Autor(es): dc.creatorDelazari dos Santos, Lucilene [UNESP]-
Autor(es): dc.creatorDal-Pai-Silva, Maeli [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:28:30Z-
Data de disponibilização: dc.date.available2022-02-22T00:28:30Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-03-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1111/are.14456-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/199835-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/199835-
Descrição: dc.descriptionHere, we aimed to study the slow muscle of the fish Piaractus mesopotamicus submitted to 30 days of fasting (D30) followed by 1 day (D31) or 30 days of refeeding (D60). Histological analysis of fibre diameter was performed in D30 and D60. The gene expression of parvalbumin (pvalb), atrogenes (murf1a, murf1b, mafbx) and anabolic genes (igf-1, mtor) was analysed using RT-qPCR in D30, D31 and D60. The proteome was obtained by shotgun proteomics at D30 and D60, and the set of differentially expressed proteins was analysed by bioinformatics. In all experiments, the control was regularly fed fish. The histological analysis showed no changes in muscle fibre diameter. The expression of catabolic and anabolic genes was not changed, except for the downregulation of igf-1 in D30 and of mafbx in D31. The expression of pvalb was not changed in D30 and D60 but was decreased in D31. The proteomic analysis identified 169 proteins in D30 (24 upregulated and 18 downregulated) and 170 proteins in D60 (17 upregulated and 21 downregulated); many of them were related to energetic metabolism and intracellular Ca2+ homoeostasis. Overall, our results indicate that the slow-twitch muscle presented few changes upon prolonged fasting and refeeding condition.-
Descrição: dc.descriptionDepartment of Morphology Institute of Biosciences of Botucatu São Paulo State University (UNESP)-
Descrição: dc.descriptionCentre for the Studies of Venoms and Venomous Animals (CEVAP)/Graduate Program in Tropical Diseases (FMB) São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Morphology Institute of Biosciences of Botucatu São Paulo State University (UNESP)-
Descrição: dc.descriptionCentre for the Studies of Venoms and Venomous Animals (CEVAP)/Graduate Program in Tropical Diseases (FMB) São Paulo State University (UNESP)-
Formato: dc.format1101-1112-
Idioma: dc.languageen-
Relação: dc.relationAquaculture Research-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectfish-
Palavras-chave: dc.subjectfood restriction-
Palavras-chave: dc.subjectproteomics-
Palavras-chave: dc.subjectred muscle-
Palavras-chave: dc.subjectrefeeding-
Palavras-chave: dc.subjectslow-twitch fibres-
Título: dc.titleAnabolism, catabolism and proteomic analysis in the slow-twitch muscle of pacu (Piaractus mesopotamicus) submitted to prolonged fasting followed by refeeding-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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