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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | University of Florida | - |
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.contributor | University of Florida Health Science Center | - |
Autor(es): dc.contributor | Federal University of Goias (UFG) | - |
Autor(es): dc.contributor | East Carolina University | - |
Autor(es): dc.creator | Rocha, Fernanda R.G. [UNESP] | - |
Autor(es): dc.creator | Delitto, Andrea E. | - |
Autor(es): dc.creator | de Souza, Joao A Chaves | - |
Autor(es): dc.creator | Maldonado, Laura A.G. [UNESP] | - |
Autor(es): dc.creator | Wallet, Shannon M. | - |
Autor(es): dc.creator | Rossa, Carlos [UNESP] | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:28:24Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:28:24Z | - |
Data de envio: dc.date.issued | 2020-12-11 | - |
Data de envio: dc.date.issued | 2020-12-11 | - |
Data de envio: dc.date.issued | 2019-12-31 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1016/j.imbio.2019.10.004 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/199817 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/199817 | - |
Descrição: dc.description | There is virtually no information on the role of NLRC4 inflammasome on bone resorption and inflammation associated with periodontitis. Bacterial-associated experimental periodontitis was induced in wild-type (WT) and Nlrc4-KO C57BL/6 mice. 3 μL of a 1 × 109 UFC/mL PBS suspension of heat-killed Gram-negative bacteria were injected (3x/week for 4 weeks) directly into the gingival tissues of WT and Nlrc4-KO mice (n = 6/genotype). Control animals were injected bilaterally (3x/week for 4 weeks) in the same sites with the same volume of the PBS vehicle. Alveolar bone resorption was quantified by μCT. Inflammatory infiltrate in the gingival tissues was assessed qualitatively in H&E-stained slides and by the detection of a pan-leukocyte marker (CD45) and a neutrophil marker (Ly6G) using immunofluorescence. Modulation of Rankl, Mmp-13, Tnf-a, Il-6 and Il-10 expression in the gingival tissues was determined by RT-qPCR. Osteoclastogenesis was assessed in vivo by biochemical staining for TRAP. The relevance of NLRC4 for RANKL-induced osteoclastic differentiation and activity was investigated in vitro using bone marrow-derived macrophages from WT and Nlrc4-KO mice. Bone resorption was significantly greater in Nlrc4-KO mice; however there were no differences between WT and Nlrc4-KO mice on osteoclast numbers and on the inflammatory infiltrate. In vitro, osteoclast activity was significantly enhanced in Nlrc4-deficient macrophages; whereas RANKL-induced differentiation was not affected. Expression of the selected candidate genes was also similarly increased by the induction of experimental periodontal disease, except for the expression of Tnf-alpha and Il-10, which was already significantly higher in the gingival tissues of Nlrc4-KO mice. We conclude that NLRC4 inflammasome has a protective role on inflammatory bone resorption in this experimental model. Furthermore, the bone-sparing effect may be related with the modulation of osteoclast activity. | - |
Descrição: dc.description | Department of Oral Biology College of Dentistry University of Florida | - |
Descrição: dc.description | Department of Diagnosis and Surgery UNESP-State University of Sao Paulo School of Dentistry at Araraquara | - |
Descrição: dc.description | Department of Physical Therapy University of Florida Health Science Center | - |
Descrição: dc.description | Department of Stomatology School of Dentistry Federal University of Goias (UFG) | - |
Descrição: dc.description | Department of Foundational Sciences College of Dental Medicine East Carolina University | - |
Descrição: dc.description | Department of Diagnosis and Surgery UNESP-State University of Sao Paulo School of Dentistry at Araraquara | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Immunobiology | - |
???dc.source???: dc.source | Scopus | - |
Palavras-chave: dc.subject | Bone resorption | - |
Palavras-chave: dc.subject | Inflammasomes | - |
Palavras-chave: dc.subject | Inflammation | - |
Palavras-chave: dc.subject | Periodontal diseases | - |
Título: dc.title | NLRC4 inflammasome has a protective role on inflammatory bone resorption in a murine model of periodontal disease | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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