NLRC4 inflammasome has a protective role on inflammatory bone resorption in a murine model of periodontal disease

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversity of Florida-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversity of Florida Health Science Center-
Autor(es): dc.contributorFederal University of Goias (UFG)-
Autor(es): dc.contributorEast Carolina University-
Autor(es): dc.creatorRocha, Fernanda R.G. [UNESP]-
Autor(es): dc.creatorDelitto, Andrea E.-
Autor(es): dc.creatorde Souza, Joao A Chaves-
Autor(es): dc.creatorMaldonado, Laura A.G. [UNESP]-
Autor(es): dc.creatorWallet, Shannon M.-
Autor(es): dc.creatorRossa, Carlos [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:28:24Z-
Data de disponibilização: dc.date.available2022-02-22T00:28:24Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2019-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.imbio.2019.10.004-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/199817-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/199817-
Descrição: dc.descriptionThere is virtually no information on the role of NLRC4 inflammasome on bone resorption and inflammation associated with periodontitis. Bacterial-associated experimental periodontitis was induced in wild-type (WT) and Nlrc4-KO C57BL/6 mice. 3 μL of a 1 × 109 UFC/mL PBS suspension of heat-killed Gram-negative bacteria were injected (3x/week for 4 weeks) directly into the gingival tissues of WT and Nlrc4-KO mice (n = 6/genotype). Control animals were injected bilaterally (3x/week for 4 weeks) in the same sites with the same volume of the PBS vehicle. Alveolar bone resorption was quantified by μCT. Inflammatory infiltrate in the gingival tissues was assessed qualitatively in H&E-stained slides and by the detection of a pan-leukocyte marker (CD45) and a neutrophil marker (Ly6G) using immunofluorescence. Modulation of Rankl, Mmp-13, Tnf-a, Il-6 and Il-10 expression in the gingival tissues was determined by RT-qPCR. Osteoclastogenesis was assessed in vivo by biochemical staining for TRAP. The relevance of NLRC4 for RANKL-induced osteoclastic differentiation and activity was investigated in vitro using bone marrow-derived macrophages from WT and Nlrc4-KO mice. Bone resorption was significantly greater in Nlrc4-KO mice; however there were no differences between WT and Nlrc4-KO mice on osteoclast numbers and on the inflammatory infiltrate. In vitro, osteoclast activity was significantly enhanced in Nlrc4-deficient macrophages; whereas RANKL-induced differentiation was not affected. Expression of the selected candidate genes was also similarly increased by the induction of experimental periodontal disease, except for the expression of Tnf-alpha and Il-10, which was already significantly higher in the gingival tissues of Nlrc4-KO mice. We conclude that NLRC4 inflammasome has a protective role on inflammatory bone resorption in this experimental model. Furthermore, the bone-sparing effect may be related with the modulation of osteoclast activity.-
Descrição: dc.descriptionDepartment of Oral Biology College of Dentistry University of Florida-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery UNESP-State University of Sao Paulo School of Dentistry at Araraquara-
Descrição: dc.descriptionDepartment of Physical Therapy University of Florida Health Science Center-
Descrição: dc.descriptionDepartment of Stomatology School of Dentistry Federal University of Goias (UFG)-
Descrição: dc.descriptionDepartment of Foundational Sciences College of Dental Medicine East Carolina University-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery UNESP-State University of Sao Paulo School of Dentistry at Araraquara-
Idioma: dc.languageen-
Relação: dc.relationImmunobiology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBone resorption-
Palavras-chave: dc.subjectInflammasomes-
Palavras-chave: dc.subjectInflammation-
Palavras-chave: dc.subjectPeriodontal diseases-
Título: dc.titleNLRC4 inflammasome has a protective role on inflammatory bone resorption in a murine model of periodontal disease-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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