43 kDa glycoprotein (gp43) from paracoccidioides brasiliensis induced IL-17A and PGE2 production by human polymorphonuclear neutrophils: Involvement of TLR2 and TLR4

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Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversity Center of Adamantina-
Autor(es): dc.contributorUniversidade Federal de São Paulo (UNIFESP)-
Autor(es): dc.contributorVanderbilt University Medical Center-
Autor(es): dc.creatorGardizani, Taiane Priscila [UNESP]-
Autor(es): dc.creatorDella Coletta, Amanda Manoel [UNESP]-
Autor(es): dc.creatorRomagnoli, Graziela Gorete [UNESP]-
Autor(es): dc.creatorPuccia, Rosana-
Autor(es): dc.creatorSerezani, Ana Paula Moreira-
Autor(es): dc.creatorDe Campos Soares, Ângela Maria Victoriano [UNESP]-
Autor(es): dc.creatorDias-Melicio, Luciane Alarcão [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:28:23Z-
Data de disponibilização: dc.date.available2022-02-22T00:28:23Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2019-01-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1155/2019/1790908-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/199812-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/199812-
Descrição: dc.descriptionThe glycoprotein gp43 is the major antigenic/diagnostic component of Paracoccidioides brasiliensis, one of the etiologic agents of paracoccidioidomycosis (PCM). Gp43 has protective roles in mice, but due to adhesive properties, this glycoprotein has also been associated with immune evasion mechanisms. The present study evaluated gp43 interaction in vitro with Toll-like receptors 2 and 4 (TLR2 and TLR4) present in polymorphonuclear neutrophils (PMNs) from healthy human individuals and the consequent modulation of the immune response through the expression and release of cytokines and eicosanoids. PMNs were incubated in the absence or presence of monoclonal antibodies anti-TLR2 and anti-TLR4 (individually or in combination) before gp43 stimulation. Then, PMNs were analyzed for the expression of both surface receptors and the detection of intracytoplasmic IL-17A and IL-4 using flow cytometry, while the production of PGE2, LTB4, IL-6, IL-10, IL-12, IFN-γ, and TNF-α was evaluated in the supernatants by enzyme-linked immunosorbent assay (ELISA). Our results showed that gp43 increased TLR2 and TLR4 expression by PMNs and induced PGE2 and IL-17A via TLR4 and TLR2, respectively. Thus, our data suggest that gp43 from P. brasiliensis might modulate host susceptibility to the fungal infection by affecting PGE2 and IL-17A production.-
Descrição: dc.descriptionLaboratory of Immunopathology and Infectious Agents (LIAI) Experimental Research Unit (UNIPEX) Medical School of Botucatu (FMB) São Paulo State University (UNESP), Av. Professor Montenegro, s/n, Distrito de Rubião Júnior-
Descrição: dc.descriptionDepartment of Medicine University Center of Adamantina, Av. Francisco Bellusci, 1000, Distrito Industrial Otavio Gacazzi-
Descrição: dc.descriptionDepartment of Microbiology and Immunology Institute of Biosciences (IB) São Paulo State University (UNESP), Av. Professor Montenegro, s/n, Distrito de Rubião Júnior-
Descrição: dc.descriptionDepartamento de Microbiologia Imunologia e Parasitologia Escola Paulista de Medicina-Universidade Federal de São Paulo, Rua Botucatu, 862-
Descrição: dc.descriptionDivision of Allergy Pulmonary and Critical Care Medicine Department of Medicine Vanderbilt University Medical Center-
Descrição: dc.descriptionDepartment of Pathology Medical School of Botucatu (FMB) São Paulo State University (UNESP), Av. Professor Montenegro, s/n, Distrito de Rubião Júnior-
Descrição: dc.descriptionLaboratory of Immunopathology and Infectious Agents (LIAI) Experimental Research Unit (UNIPEX) Medical School of Botucatu (FMB) São Paulo State University (UNESP), Av. Professor Montenegro, s/n, Distrito de Rubião Júnior-
Descrição: dc.descriptionDepartment of Microbiology and Immunology Institute of Biosciences (IB) São Paulo State University (UNESP), Av. Professor Montenegro, s/n, Distrito de Rubião Júnior-
Descrição: dc.descriptionDepartment of Pathology Medical School of Botucatu (FMB) São Paulo State University (UNESP), Av. Professor Montenegro, s/n, Distrito de Rubião Júnior-
Idioma: dc.languageen-
Relação: dc.relationJournal of Immunology Research-
???dc.source???: dc.sourceScopus-
Título: dc.title43 kDa glycoprotein (gp43) from paracoccidioides brasiliensis induced IL-17A and PGE2 production by human polymorphonuclear neutrophils: Involvement of TLR2 and TLR4-
Tipo de arquivo: dc.typelivro digital-
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