Satellite DNA content of B chromosomes in the characid fish Characidium gomesi supports their origin from sex chromosomes

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorFunepe-
Autor(es): dc.contributorUniversidad de Granada-
Autor(es): dc.contributorUppsala University-
Autor(es): dc.contributorUniversidade Federal Rural do Rio de Janeiro-
Autor(es): dc.contributorUniversidad de La Serena-
Autor(es): dc.creatorSerrano-Freitas, Érica A. [UNESP]-
Autor(es): dc.creatorSilva, Duílio M. Z. A. [UNESP]-
Autor(es): dc.creatorRuiz-Ruano, Francisco J.-
Autor(es): dc.creatorUtsunomia, Ricardo-
Autor(es): dc.creatorAraya-Jaime, Cristian-
Autor(es): dc.creatorOliveira, Claudio [UNESP]-
Autor(es): dc.creatorCamacho, Juan Pedro M.-
Autor(es): dc.creatorForesti, Fausto [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:28:22Z-
Data de disponibilização: dc.date.available2022-02-22T00:28:22Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2019-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s00438-019-01615-2-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/199807-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/199807-
Descrição: dc.descriptionThe origin of supernumerary (B) chromosomes is clearly conditioned by their ancestry from the standard (A) chromosomes. Sequence similarity between A and B chromosomes is thus crucial to determine B chromosome origin. For this purpose, we compare here the DNA sequences from A and B chromosomes in the characid fish Characidium gomesi using two main approaches. First, we found 59 satellite DNA (satDNA) families constituting the satellitome of this species and performed FISH analysis for 18 of them. This showed the presence of six satDNAs on the B chromosome: one shared with sex chromosomes and autosomes, two shared with sex chromosomes, one shared with autosomes and two being B-specific. This indicated that B chromosomes most likely arose from the sex chromosomes. Our second approach consisted of the analysis of five repetitive DNA families: 18S and 5S ribosomal DNA (rDNA), the H3 histone gene, U2 snDNA and the most abundant satDNA (CgoSat01-184) on DNA obtained from microdissected B chromosomes and from B-lacking genomes. PCR and sequence analysis of these repetitive sequences was successful for three of them (5S rDNA, H3 histone gene and CgoSat01-184), and sequence comparison revealed that DNA sequences obtained from the B chromosomes displayed higher identity with C. gomesi genomic DNA than with those obtained from other Characidium species. Taken together, our results support the intraspecific origin of B chromosomes in C. gomesi and point to sex chromosomes as B chromosome ancestors, which raises interesting prospects for future joint research on the genetic content of sex and B chromosomes in this species.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartamento de Morfologia Instituto de Biociências de Botucatu Universidade Estadual Paulista UNESP, Distrito de Rubião Junior-
Descrição: dc.descriptionCentro de Ciências Biológicas e da Saúde Fundação Educacional de Penápolis Funepe-
Descrição: dc.descriptionDepartamento de Genética Universidad de Granada-
Descrição: dc.descriptionDepartment of Ecology and Genetics Evolutionary Biology Centre Uppsala University-
Descrição: dc.descriptionDepartamento de Genética Instituto de Ciências Biológicas e da Saúde ICBS Universidade Federal Rural do Rio de Janeiro-
Descrição: dc.descriptionInstituto de Investigación Multidisciplinar en Ciencia y Tecnología Universidad de La Serena-
Descrição: dc.descriptionLaboratorio de Genética y Citogenética Vegetal Departamento de Biología Universidad de La Serena-
Descrição: dc.descriptionDepartamento de Morfologia Instituto de Biociências de Botucatu Universidade Estadual Paulista UNESP, Distrito de Rubião Junior-
Descrição: dc.descriptionFAPESP: 2013/02143-3-
Formato: dc.format195-207-
Idioma: dc.languageen-
Relação: dc.relationMolecular Genetics and Genomics-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectFluorescence in situ hybridization-
Palavras-chave: dc.subjectNext-generation sequencing-
Palavras-chave: dc.subjectRepeatExplorer-
Palavras-chave: dc.subjectSatellitome-
Título: dc.titleSatellite DNA content of B chromosomes in the characid fish Characidium gomesi supports their origin from sex chromosomes-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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