Function of cryopreserved cat ovarian tissue after autotransplantation

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade de Brasília (UnB)-
Autor(es): dc.contributorUniversité Catholique de Louvain-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorCiência e Tecnologia do Amazonas-
Autor(es): dc.creatorVilela, Janice M. V.-
Autor(es): dc.creatorLeonel, Ellen C. R. [UNESP]-
Autor(es): dc.creatorGonçalves, Liudimila P.-
Autor(es): dc.creatorPaiva, Raísa E. G.-
Autor(es): dc.creatorAmaral, Rodrigo S.-
Autor(es): dc.creatorAmorim, Christiani A.-
Autor(es): dc.creatorLucci, Carolina M.-
Data de aceite: dc.date.accessioned2022-02-22T00:28:17Z-
Data de disponibilização: dc.date.available2022-02-22T00:28:17Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2019-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/ani9121065-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/199773-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/199773-
Descrição: dc.descriptionThe aim of this study was to assess a slow-freezing protocol of cat ovarian tissue cryopreservation using autotransplantation. Four adult queens were ovariohysterectomized and the ovaries were fragmented and cryopreserved. After one week, the grafts were thawed and autografted to the subcutaneous tissue of the dorsal neck of each queen, then randomly removed after 7, 14, 28, 49, and 63 days after transplantation. Percentages of morphologically normal primordial and growing follicles (MNFs) were 88% and 97%, respectively, in fresh tissue samples (fresh controls), and 74% and 100%, respectively, immediately after thawing (cryo D0). No MNFs were found after 49 days of transplantation. In both fresh control and cryo D0 fragments, granulosa cells were frequently in proliferation. Two morphologically normal antral follicles were detected in one queen on Day 28 post-transplantation. Connective tissue fibers increased, suggesting replacement of active ovarian cortex by fibrous tissue. Tissue vascularization was observed at 7 days after grafting, and wide blood vessels were clearly visible on Days 49 and 63. In conclusion, although follicular survival was low after cryopreservation and grafting of cat ovarian tissue, follicles were able to develop up to the antral stage, which is an encouraging outcome.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionWallonie-Bruxelles International-
Descrição: dc.descriptionDepartamento de Ciências Fisiológicas Instituto de Ciências Biológicas Campus Universitário Darcy Ribeiro Universidade de Brasília, Distrito Federal-
Descrição: dc.descriptionInstitut de Recherche Expérimentale et Clinique Pôle de Recherche en Gynécologie Université Catholique de Louvain, Avenue Mounier, 52 bte B1.52.02-
Descrição: dc.descriptionDepartamento de Biologia Instituto de Biociências Letras e Ciências Exatas (IBILCE) Universidade Estadual Paulista (UNESP), Rua Cristóvão Colombo, 2265-
Descrição: dc.descriptionInstituto Federal de Educação Ciência e Tecnologia do Amazonas Campus Manaus Zona Leste-IFAM/CMZL, Avenida Cosme Ferreira, 8045-
Descrição: dc.descriptionDepartamento de Biologia Instituto de Biociências Letras e Ciências Exatas (IBILCE) Universidade Estadual Paulista (UNESP), Rua Cristóvão Colombo, 2265-
Descrição: dc.descriptionCAPES: #013/14-
Descrição: dc.descriptionWallonie-Bruxelles International: #013/14-
Descrição: dc.descriptionCAPES: 001-
Idioma: dc.languageen-
Relação: dc.relationAnimals-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAntral follicle-
Palavras-chave: dc.subjectCryopreservation-
Palavras-chave: dc.subjectFelids-
Palavras-chave: dc.subjectOvary-
Palavras-chave: dc.subjectTransplant-
Título: dc.titleFunction of cryopreserved cat ovarian tissue after autotransplantation-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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