Angus sire field fertility and in vitro sperm characteristics following use of different sperm insemination doses in Brazilian beef cattle

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Autor(es): dc.contributorUniversity of Idaho-
Autor(es): dc.contributorInc.-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorMenegatti Zoca, Saulo-
Autor(es): dc.creatorShafii, Bahman-
Autor(es): dc.creatorPrice, William-
Autor(es): dc.creatorUtt, Matthew-
Autor(es): dc.creatorHarstine, Bo-
Autor(es): dc.creatorMcDonald, Kristina-
Autor(es): dc.creatorCruppe, Leandro-
Autor(es): dc.creatorDeJarnette, Mel-
Autor(es): dc.creatorPeters, Lon-
Autor(es): dc.creatorMoraes Vasconcelos, Jose Luiz [UNESP]-
Autor(es): dc.creatorDalton, Joseph-
Data de aceite: dc.date.accessioned2022-02-22T00:28:16Z-
Data de disponibilização: dc.date.available2022-02-22T00:28:16Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-04-15-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.theriogenology.2019.11.021-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/199764-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/199764-
Descrição: dc.descriptionThe primary objective was to determine if Angus bull fertility varied by number of sperm inseminated. A secondary objective was to characterize the potential impact of random variation on fertility using two identical sperm per dose treatments, which differed only in straw color. Computer-assisted sperm analysis (CASA) and flow cytometry (FC) were used to identify post-thaw sperm characteristics associated with field fertility differences between bulls. Ejaculates from five Angus bulls were collected, extended, and cryopreserved at 10, 20, 20 or 40 × 106 sperm per dose in color-coded 0.5-mL French straws. Multiparous cows (n = 4866) from ten Brazilian farms were synchronized for first-service timed artificial insemination (TAI). Bull identification and straw color were recorded at TAI. Pregnancy per TAI (P/TAI) did not differ between sperm doses (43.8, 45.3, 43.8 and 47.1% for 10, 20, 20 or 40 × 106 sperm respectively; P = 0.31) nor was there an interaction between bull and dose (P = 0.53). The P/TAI differed between bulls and ranged from 40.7 to 48.1% (P < 0.01). The overall P/TAI between the two control groups were not different (45.3 vs 43.8%); however, the numerical variation within bull ranged from 0.5 to 4.9 percentage points. Numerous CASA and FC post-thaw sperm characteristics differed among bulls (P < 0.05), but these characteristics did not explain the fertility difference between bulls. Principal component analysis provided a multivariate description of the CASA and FC data, where three principal components (Prin1, Prin2, and Prin3) accounted for a combined total of 88.7% of the data variability. The primary components of each PCA axis were flow cytometric measures of sperm viability and DNA fragmentation (Prin1), and CASA-derived sperm movement patterns (Prin2) and motility (Prin3); however, the relative influence of these characteristics varied by bull. Although fertility differences between bulls were detected, neither sperm per dose nor post-thaw in vitro sperm analyses (CASA and FC) were able to explain the observed differences in field fertility between bulls, further illustrating the difficulties in predicting bull fertility.-
Descrição: dc.descriptionAnimal and Veterinary Science Department University of Idaho-
Descrição: dc.descriptionStatistical Programs College of Agricultural and Life Sciences University of Idaho-
Descrição: dc.descriptionSelect Sires Inc.-
Descrição: dc.descriptionDepartamento de Produçáo Animal Faculdade de Medicina Veterinária e Zootecnia-UNESP, São Paulo-
Descrição: dc.descriptionDepartamento de Produçáo Animal Faculdade de Medicina Veterinária e Zootecnia-UNESP, São Paulo-
Formato: dc.format146-153-
Idioma: dc.languageen-
Relação: dc.relationTheriogenology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBeef cattle-
Palavras-chave: dc.subjectBull fertility-
Palavras-chave: dc.subjectComputer-assisted sperm analysis-
Palavras-chave: dc.subjectFlow cytometry-
Palavras-chave: dc.subjectSperm dose-
Título: dc.titleAngus sire field fertility and in vitro sperm characteristics following use of different sperm insemination doses in Brazilian beef cattle-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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