Phthalocyanine-loaded nanostructured lipid carriers functionalized with folic acid for photodynamic therapy

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorState University of Paraíba (UEPB)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.contributorUniversity of Ulster-
Autor(es): dc.creatorOshiro-Junior, João A.-
Autor(es): dc.creatorSato, Mariana Rillo [UNESP]-
Autor(es): dc.creatorBoni, Fernanda Isadora [UNESP]-
Autor(es): dc.creatorSantos, Karen Loraine Macena-
Autor(es): dc.creatorde Oliveira, Kleber Thiago-
Autor(es): dc.creatorde Freitas, Laura Marise [UNESP]-
Autor(es): dc.creatorFontana, Carla Raquel [UNESP]-
Autor(es): dc.creatorNicholas, Dean-
Autor(es): dc.creatorMcHale, Anthony-
Autor(es): dc.creatorCallan, John F.-
Autor(es): dc.creatorChorilli, Marlus [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:28:07Z-
Data de disponibilização: dc.date.available2022-02-22T00:28:07Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-03-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.msec.2019.110462-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/199714-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/199714-
Descrição: dc.descriptionBreast cancer is a serious public health problem that causes thousands of deaths annually. Chemotherapy continues to play a central role in the management of breast cancer but is associated with extreme off-target toxicity. Therefore, treatments that directly target the tumor and display reduced susceptibility to resistance could improve the outcome and quality of life for patients suffering from this disease. Photodynamic therapy is a targeted treatment based on the use of light to activate a photosensitizer (PS) that then interacts with molecular oxygen and other biochemical substrates to generate cytotoxic levels of Reactive Oxygen Species. Currently approved PS also tends to have poor aqueous solubility that can cause problems when delivered intravenously. In order to circumvent this limitation, in this manuscript, we evaluate the potential of a phthalocyanine-loaded nanostructured lipid carrier (NLC) functionalized with folic acid (FA). To prepare the FA labelled NLC, the polymer PF127 was first esterified with FA and emulsified with an oil phase containing polyoxyethylene 40 stearate, capric/caprylic acid triglycerides, ethoxylated hydrogenated castor oil 40 and the PS zinc phthalocyanine. The resulting PS loaded FA-NLC had a hydrodynamic diameter of 180 nm and were stable in suspension for >90 days. Interestingly, the amount of singlet oxygen generated upon light activation for the PS loaded FA-NLC was substantially higher than the free PS, yet at a lower PS concentration. The PS was released from the NLC in a sustained manner with 4.13 ± 0.58% and 27.7 ± 3.16% after 30 min and 7 days, respectively. Finally, cytotoxicity assays showed that NLC in the concentrations of 09.1 μM of PS present non-toxic with >80 ± 6.8% viable and after 90 s of the light-exposed the results show a statistically significant decrease in cell viability (57 ± 4%). The results obtained allow us to conclude that the functionalized NLC incorporated with PS associated with the PDT technique have characteristics that make them potential candidates for the alternative treatment of breast cancer.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionUlster University-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionGraduation Program in Pharmaceutical Sciences Center for Biological and Health Sciences State University of Paraíba (UEPB)-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Pharmaceutical Sciences Araraquara Department of Drugs and Medicines, Rodovia Araraquara-Jaú, km 1-
Descrição: dc.descriptionDepartamento de Química Universidade Federal de São Carlos-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Pharmaceutical Sciences Araraquara Department of Clinical Analyses, Rodovia Araraquara-Jaú, km 1-
Descrição: dc.descriptionBiomedical Sciences Research Institute University of Ulster-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Pharmaceutical Sciences Araraquara Department of Drugs and Medicines, Rodovia Araraquara-Jaú, km 1-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Pharmaceutical Sciences Araraquara Department of Clinical Analyses, Rodovia Araraquara-Jaú, km 1-
Descrição: dc.descriptionFAPESP: 2014/50928-2-
Descrição: dc.descriptionUlster University: 2016/11198-4-
Descrição: dc.descriptionUlster University: 2018/17573-7-
Descrição: dc.descriptionCNPq: 465687/2014-8-
Idioma: dc.languageen-
Relação: dc.relationMaterials Science and Engineering C-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBreast cancer-
Palavras-chave: dc.subjectFolic acid-
Palavras-chave: dc.subjectFunctionalization-
Palavras-chave: dc.subjectNanostructured lipid carriers-
Palavras-chave: dc.subjectPhotodynamic therapy-
Palavras-chave: dc.subjectPhthalocyanines-
Título: dc.titlePhthalocyanine-loaded nanostructured lipid carriers functionalized with folic acid for photodynamic therapy-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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