Immunization with recombinant enolase of Sporothrix spp. (rSsEno) confers effective protection against sporotrichosis in mice

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Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorPontificia Universidad Católica de Valparaíso-
Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.contributorLaboratory of Clinical Research on Dermatozoonoses in Domestic Animals (Lapclin-Dermzoo)-
Autor(es): dc.creatorPortuondo, Deivys Leandro [UNESP]-
Autor(es): dc.creatorDores-Silva, Paulo Roberto-
Autor(es): dc.creatorFerreira, Lucas Souza [UNESP]-
Autor(es): dc.creatorde Oliveira, Carlos S.-
Autor(es): dc.creatorTéllez-Martínez, Damiana [UNESP]-
Autor(es): dc.creatorMarcos, Caroline Maria [UNESP]-
Autor(es): dc.creatorde Aguiar Loesch, Maria Luiza [UNESP]-
Autor(es): dc.creatorGuzmán, Fanny-
Autor(es): dc.creatorGava, Lisandra M.-
Autor(es): dc.creatorBorges, Júlio César-
Autor(es): dc.creatorPereira, Sandro Antonio-
Autor(es): dc.creatorBatista-Duharte, Alexander [UNESP]-
Autor(es): dc.creatorCarlos, Iracilda Zeppone [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:28:04Z-
Data de disponibilização: dc.date.available2022-02-22T00:28:04Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2019-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1038/s41598-019-53135-z-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/199694-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/199694-
Descrição: dc.descriptionIn recent years, research has focused on the immunoreactive components of the Sporothrix schenckii cell wall that can be relevant targets for preventive and therapeutic vaccines against sporotrichosis, an emergent worldwide mycosis. In a previous study, we identified a 47-kDa enolase as an immunodominant antigen in mice vaccinated with an adjuvanted mixture of S. schenckii cell wall proteins. Here, we sought to assess the protective potential of a Sporothrix spp. recombinant enolase (rSsEno) formulated with or without the adjuvant Montanide Pet-GelA (PGA) against the S. brasiliensis infection in mice. Mice that were immunized with rSsEno plus PGA showed increased antibody titters against rSsEno and increased median survival time when challenged with S. brasiliensis as compared with mice that had not been immunized or that were immunized with rSsEno alone. Immunization with rSsEno plus PGA induced a predominantly T-helper 1 cytokine pattern after in vitro stimulation of splenic cells with rSsEno: elevated levels of IFN-γ and IL-2, as well as of other cytokines involved in host defense against sporotrichosis, such as TNF-alpha, IL-6, and IL-4. Furthermore, we show for the first time the presence of enolase in the cell wall of both S. schenckii and S. brasiliensis. As a whole, our results suggest that enolase could be used as a potential antigenic target for vaccinal purposes against sporotrichosis.-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Pharmaceutical Sciences Department of Clinical Analysis-
Descrição: dc.descriptionSão Carlos Institute of Chemistry University of São Paulo, P.O. Box 780-
Descrição: dc.descriptionNúcleo Biotecnológico de Curauma (NBC) Pontificia Universidad Católica de Valparaíso-
Descrição: dc.descriptionCenter of Biological and Health Sciences Federal University of São Carlos-
Descrição: dc.descriptionOswaldo Cruz Foundation (Fiocruz) Evandro Chagas National Institute of Infectious Diseases (INI) Laboratory of Clinical Research on Dermatozoonoses in Domestic Animals (Lapclin-Dermzoo)-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Pharmaceutical Sciences Department of Clinical Analysis-
Idioma: dc.languageen-
Relação: dc.relationScientific Reports-
???dc.source???: dc.sourceScopus-
Título: dc.titleImmunization with recombinant enolase of Sporothrix spp. (rSsEno) confers effective protection against sporotrichosis in mice-
Tipo de arquivo: dc.typelivro digital-
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