Fridericia platyphylla (Cham.) L.G. Lohmann root extract exerts cytotoxic and antiproliferative effects on gastric tumor cells and downregulates BCL-XL, BIRC5, and MET genes

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade Estadual de Londrina (UEL)-
Autor(es): dc.contributorSciences and Letters of Ribeirão Preto (FFCLRP)-
Autor(es): dc.contributorFederal University of Maranhão (UFMA)-
Autor(es): dc.creatorSerpeloni, J. M. [UNESP]-
Autor(es): dc.creatorSpecian, A. F.L.-
Autor(es): dc.creatorRibeiro, D. L.-
Autor(es): dc.creatorBenício, L. M.-
Autor(es): dc.creatorNunes, H. L.-
Autor(es): dc.creatorFranchi, L. P.-
Autor(es): dc.creatorRocha, C. Q.-
Autor(es): dc.creatorVilegas, W. [UNESP]-
Autor(es): dc.creatorVaranda, E. A. [UNESP]-
Autor(es): dc.creatorCólus, I. M.S. [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:28:00Z-
Data de disponibilização: dc.date.available2022-02-22T00:28:00Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-03-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1177/0960327119888261-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/199669-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/199669-
Descrição: dc.descriptionFridericia platyphylla (Cham.) L.G. Lohmann (FP) has cytotoxic, anti-inflammatory, and analgesic properties. We aimed to characterize the cytotoxic and antiproliferative effects of FP extract on normal (GAS) and tumor-derived (ACP02 and HepG2) cell lines. The effective concentrations (EC50s) by tetrazolium bromide assay (MTT) were 56.16, 43.68, and 42.57 µg mL−1 and 69.38, 41.73, and 52.39 µg mL−1 by neutral red assay for GAS, ACP02, and HepG2 cells, respectively. The extract decreased nuclear division indices, which was not reflected in cell proliferation curves. Flow cytometric analyses showed that even 30 µg mL−1 extract (shown to be noncytotoxic by MTT assay) increased the sub-G1 population, indicating cell death due to apoptosis and necrosis. A cytokinesis-block micronucleus cytome assay showed that 30 µg mL−1 of the extract increased the frequency of nuclear buds in tumor cells. Real-time quantitative polymerase chain reaction showed CCND1 upregulation in doxorubicin-treated GAS cells and BCL-XL, BIRC5, and MET downregulation in 5 or 30 µg mL−1 in FP extract-treated ACP02 cells. In conclusion, FP extract modulated apoptosis- and cell cycle-related genes and presented selective cytotoxicity toward tumor cells that deserves further investigation by testing other cell types. Our results demonstrated that even medicinal plants exert adverse effects depending on the extract concentrations used and tissues investigated.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa e ao Desenvolvimento Científico e Tecnológico do Maranhão-
Descrição: dc.descriptionLaboratory of Mutagenesis Department of Biological Sciences Faculty of Pharmaceutical Sciences São Paulo State University (UNESP)-
Descrição: dc.descriptionLaboratory of Mutagenesis and Oncogenetics Department of General Biology Center of Biological Sciences State University of Londrina (UEL)-
Descrição: dc.descriptionLaboratory of Cytogenetics and Mutagenesis Department of Biology Faculty of Philosophy Sciences and Letters of Ribeirão Preto (FFCLRP)-
Descrição: dc.descriptionLaboratory of Advanced Studies in Phytomedicines Department of Chemistry Federal University of Maranhão (UFMA)-
Descrição: dc.descriptionCampus Litoral Paulista São Paulo State University (UNESP)-
Descrição: dc.descriptionLaboratory of Mutagenesis Department of Biological Sciences Faculty of Pharmaceutical Sciences São Paulo State University (UNESP)-
Descrição: dc.descriptionCampus Litoral Paulista São Paulo State University (UNESP)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa e ao Desenvolvimento Científico e Tecnológico do Maranhão: 00863/16-
Formato: dc.format338-354-
Idioma: dc.languageen-
Relação: dc.relationHuman and Experimental Toxicology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAntiproliferative-
Palavras-chave: dc.subjectapoptosis-
Palavras-chave: dc.subjectFridericia platyphylla-
Palavras-chave: dc.subjectgastric cells-
Palavras-chave: dc.subjectNBUDs-
Título: dc.titleFridericia platyphylla (Cham.) L.G. Lohmann root extract exerts cytotoxic and antiproliferative effects on gastric tumor cells and downregulates BCL-XL, BIRC5, and MET genes-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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