Portable Laboratory Platform with Electrochemical Biosensors for Immunodiagnostic of Hepatitis C Virus

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Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorDe Campos Da Costa, Joao Paulo-
Autor(es): dc.creatorBastos, Wagner Benicio-
Autor(es): dc.creatorDa Costa, Paulo Inacio [UNESP]-
Autor(es): dc.creatorZaghete, Maria Aparecida [UNESP]-
Autor(es): dc.creatorLongo, Elson-
Autor(es): dc.creatorCarmo, Joao Paulo-
Data de aceite: dc.date.accessioned2022-02-22T00:27:40Z-
Data de disponibilização: dc.date.available2022-02-22T00:27:40Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2019-11-14-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1109/JSEN.2019.2930957-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/199544-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/199544-
Descrição: dc.descriptionThis paper presents a portable laboratory platform (PLP) with electrochemical amperometric biosensors for the detection of hepatitis C virus (HCV). This PLP is composed by a controller unit, a Bluetooth module to provide communication with mobile devices, and a potentiostat circuit to perform cyclic voltammetry. The Bluetooth communication allows the mobile devices to set up the parameters, e.g., a cycling voltage in the range ±1.5 V, a scan rate in the range 5-1000 mV-1 with a step potential of 2.318 mV, and up to 4000 number of scan cycles. The electrochemical sensors were functionalized with cystamine (C4H12N2S2) and glutaraldehyde (C5H8O2) to make the covalent binding with the recombinant core-HCV protein. The experimental results have revealed a positive detection of HCV, using a redox solution of 5 mmol.L-1 of potassium ferrocyanide (K3 Fe(CN)6) on 0.1 mol.L-1 of phosphate-buffered saline (PBS). The sensitivity of the immunosensor was established at the concentration of 1 ng. μ L-1 of the anti-core-HCV IgG class antibody. This PLP has a plug-and-play connection for the biosensors, and at the same time, it offers good ergonomy with easy operation due to its low dimensions (115 mm × 75 mm × 30 mm). This PLP presents a power consumption of 280.5 mW when supplied with a 3.3-V voltage source. This consumption was measured in the worst operation case, e.g., with the Bluetooth working, resulting in an average battery life of 195 min.-
Descrição: dc.descriptionDepartment of Electrical Engineering (SEL) University of São Paulo (USP)-
Descrição: dc.descriptionDepartment of Chemistry INCTMN-CDMF Federal University of São Carlos (UFSCar)-
Descrição: dc.descriptionDepartment of Clinical Analysis School of Pharmaceutical Sciences Laboratory of Clinical Immunology and Molecular Biology (LICBM) São Paulo State University (UNESP)-
Descrição: dc.descriptionInstitute of Chemistry São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Clinical Analysis School of Pharmaceutical Sciences Laboratory of Clinical Immunology and Molecular Biology (LICBM) São Paulo State University (UNESP)-
Descrição: dc.descriptionInstitute of Chemistry São Paulo State University (UNESP)-
Formato: dc.format10701-10709-
Idioma: dc.languageen-
Relação: dc.relationIEEE Sensors Journal-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectEarly diagnosis-
Palavras-chave: dc.subjectelectrochemical biosensors-
Palavras-chave: dc.subjecthepatitis C-
Palavras-chave: dc.subjectportable laboratory platform-
Título: dc.titlePortable Laboratory Platform with Electrochemical Biosensors for Immunodiagnostic of Hepatitis C Virus-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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