Ultrastructural characterization of damage in the basement membrane of facial melasma

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorEspósito, Ana Cláudia Cavalcante [UNESP]-
Autor(es): dc.creatorBrianezi, Gabrielli [UNESP]-
Autor(es): dc.creatorde Souza, Nathalia Pereira [UNESP]-
Autor(es): dc.creatorSantos, Daniela Carvalho [UNESP]-
Autor(es): dc.creatorMiot, Luciane Donida Bartoli [UNESP]-
Autor(es): dc.creatorMiot, Hélio Amante [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:27:39Z-
Data de disponibilização: dc.date.available2022-02-22T00:27:39Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-04-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s00403-019-01979-w-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/199540-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/199540-
Descrição: dc.descriptionThe pathogenesis of melasma is not fully understood, and the role of skin basement membrane zone (BMZ) alterations in disease development and the maintenance of hypermelanogenesis are also poorly known. We performed a comparative study to characterize the ultrastructural alterations that occur in BMZ in melasma and adjacent normal skin, as well as we discuss the implications of these changes in the physiopathology of the disease. Pairs of facial skin biopsies (2 mm) from 10 women with melasma and normal skin (< 2 cm apart) were processed by Transmission Electronic Microscopy or immunohistochemistry for Melan-A counterstained with Periodic acid–Schiff stain. Cytoplasmic organelles (from keratinocyte or melanocyte), BMZ damage were assessed and melanocyte counting (total and pendulous) was done. There was greater amount of cytoplasmic organelles inside basal keratinocytes and melanocytes in melasma, as well as structural damaged areas in the lamina densa (disruptions, gaps, lower density and thinning) and anchoring fibrils (lamina lucida), compared to healthy adjacent skin. Areas with pendulous melanocytes are characterized by discontinuity of BMZ ultrastructure. The prominence of cytoplasmic organelles from melanocytes and keratinocytes evidences the involvement of both cell groups in melasma. The damage in the lamina densa and lamina lucida suggest the role of upper dermis injury/repair process in the pathogenesis of the disease.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionDepartamento de Dermatologia E Radioterapia FMB-Unesp, Campus de Rubião Jr.-Unesp.-
Descrição: dc.descriptionDepartamento de Patologia FMB-Unesp-
Descrição: dc.descriptionDepartamento de Morfologia IBB-Unesp-
Descrição: dc.descriptionDepartamento de Dermatologia E Radioterapia FMB-Unesp, Campus de Rubião Jr.-Unesp.-
Descrição: dc.descriptionDepartamento de Patologia FMB-Unesp-
Descrição: dc.descriptionDepartamento de Morfologia IBB-Unesp-
Descrição: dc.descriptionFAPESP: 2012/09233-5-
Descrição: dc.descriptionCNPq: 401309/2016-9-
Formato: dc.format223-227-
Idioma: dc.languageen-
Relação: dc.relationArchives of Dermatological Research-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBasement membrane zone-
Palavras-chave: dc.subjectHistopathology-
Palavras-chave: dc.subjectLamina densa-
Palavras-chave: dc.subjectMelasma-
Palavras-chave: dc.subjectPigmentary disorders-
Palavras-chave: dc.subjectTransmission electronic microscopy-
Título: dc.titleUltrastructural characterization of damage in the basement membrane of facial melasma-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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