Treatment with roscovitine and butyrolactone i prior to in vitro maturation alters blastocyst production

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorParanaense University-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUNISA-
Autor(es): dc.creatorMaziero, Rosiara Rosária Dias [UNESP]-
Autor(es): dc.creatorGuaitolini, Carlos Renato De Freitas [UNESP]-
Autor(es): dc.creatorPaschoal, Daniela Martins [UNESP]-
Autor(es): dc.creatorCrespilho, André MacIel-
Autor(es): dc.creatorMonteiro, Bianca Andriolo [UNESP]-
Autor(es): dc.creatorLima, Jonathan Soares De-
Autor(es): dc.creatorSestari, Danielle Andressa Oliveira-
Autor(es): dc.creatorLandim-Alvarenga, Fernanda Da Cruz [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:27:35Z-
Data de disponibilização: dc.date.available2022-02-22T00:27:35Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2019-01-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1017/S0967199419000571-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/199524-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/199524-
Descrição: dc.descriptionSummaryThis study evaluated the effects of oocyte meiosis inhibitors roscovitine (ROS) and butyrolactone I (BL-I) on in vitro production of bovine embryos. Bovine oocytes were maintained in pre in vitro maturation (pre-IVM) with 25 μM ROS or 100 μM BL-I for 24 h to delay meiosis and for 24 h in in vitro maturation (IVM). Following this treatment, the nuclear maturation index was evaluated. All embryos degenerated following this procedure. In the second set of experiments, oocytes were maintained for 6 or 12 h in pre-IVM with the following three treatments: ROS (25 μM or 12.5 μM), BL-I (100 μM or 50 μM) or a combination of both drugs (6.25 μM ROS and 12.5 μM BL-I). Oocytes were cultivated for 18 or 12 h in IVM. When a meiosis-inducing agent was used during pre-IVM for 24 h, more degenerated oocytes were observed at the end of the IVM period. This effect decreased when the meiotic blocking period was reduced to 6 or 12 h. No significant differences were observed in the blastocyst production rate of oocytes in pre-IVM for 6 h with ROS, BL-I, or ROS + BL-I compared with that of the control group (P > 0.05). However, inhibition of oocytes for 12 h resulted in decreased embryo production compared with that in the controls (P < 0.05). There was no difference in the post-vitrification embryo re-expansion rate between the study groups, showing that the meiotic inhibition for 6 or 12 h did not alter the embryo cryopreservation process.-
Descrição: dc.descriptionParanaense University, Rua Leonildo Stecca-
Descrição: dc.descriptionDepartment of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Science Saõ Paulo State University UNESP FMVZ-
Descrição: dc.descriptionSanto Amaro University UNISA-
Descrição: dc.descriptionDepartment of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Science Saõ Paulo State University UNESP FMVZ-
Idioma: dc.languageen-
Relação: dc.relationZygote-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBovine-
Palavras-chave: dc.subjectEmbryo production-
Palavras-chave: dc.subjectMeiotic inhibitors-
Palavras-chave: dc.subjectNuclear maturation-
Palavras-chave: dc.subjectOocyte-
Palavras-chave: dc.subjectVitrification-
Título: dc.titleTreatment with roscovitine and butyrolactone i prior to in vitro maturation alters blastocyst production-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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