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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Paranaense University | - |
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.contributor | UNISA | - |
Autor(es): dc.creator | Maziero, Rosiara Rosária Dias [UNESP] | - |
Autor(es): dc.creator | Guaitolini, Carlos Renato De Freitas [UNESP] | - |
Autor(es): dc.creator | Paschoal, Daniela Martins [UNESP] | - |
Autor(es): dc.creator | Crespilho, André MacIel | - |
Autor(es): dc.creator | Monteiro, Bianca Andriolo [UNESP] | - |
Autor(es): dc.creator | Lima, Jonathan Soares De | - |
Autor(es): dc.creator | Sestari, Danielle Andressa Oliveira | - |
Autor(es): dc.creator | Landim-Alvarenga, Fernanda Da Cruz [UNESP] | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:27:35Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:27:35Z | - |
Data de envio: dc.date.issued | 2020-12-11 | - |
Data de envio: dc.date.issued | 2020-12-11 | - |
Data de envio: dc.date.issued | 2019-01-01 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1017/S0967199419000571 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/199524 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/199524 | - |
Descrição: dc.description | SummaryThis study evaluated the effects of oocyte meiosis inhibitors roscovitine (ROS) and butyrolactone I (BL-I) on in vitro production of bovine embryos. Bovine oocytes were maintained in pre in vitro maturation (pre-IVM) with 25 μM ROS or 100 μM BL-I for 24 h to delay meiosis and for 24 h in in vitro maturation (IVM). Following this treatment, the nuclear maturation index was evaluated. All embryos degenerated following this procedure. In the second set of experiments, oocytes were maintained for 6 or 12 h in pre-IVM with the following three treatments: ROS (25 μM or 12.5 μM), BL-I (100 μM or 50 μM) or a combination of both drugs (6.25 μM ROS and 12.5 μM BL-I). Oocytes were cultivated for 18 or 12 h in IVM. When a meiosis-inducing agent was used during pre-IVM for 24 h, more degenerated oocytes were observed at the end of the IVM period. This effect decreased when the meiotic blocking period was reduced to 6 or 12 h. No significant differences were observed in the blastocyst production rate of oocytes in pre-IVM for 6 h with ROS, BL-I, or ROS + BL-I compared with that of the control group (P > 0.05). However, inhibition of oocytes for 12 h resulted in decreased embryo production compared with that in the controls (P < 0.05). There was no difference in the post-vitrification embryo re-expansion rate between the study groups, showing that the meiotic inhibition for 6 or 12 h did not alter the embryo cryopreservation process. | - |
Descrição: dc.description | Paranaense University, Rua Leonildo Stecca | - |
Descrição: dc.description | Department of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Science Saõ Paulo State University UNESP FMVZ | - |
Descrição: dc.description | Santo Amaro University UNISA | - |
Descrição: dc.description | Department of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Science Saõ Paulo State University UNESP FMVZ | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Zygote | - |
???dc.source???: dc.source | Scopus | - |
Palavras-chave: dc.subject | Bovine | - |
Palavras-chave: dc.subject | Embryo production | - |
Palavras-chave: dc.subject | Meiotic inhibitors | - |
Palavras-chave: dc.subject | Nuclear maturation | - |
Palavras-chave: dc.subject | Oocyte | - |
Palavras-chave: dc.subject | Vitrification | - |
Título: dc.title | Treatment with roscovitine and butyrolactone i prior to in vitro maturation alters blastocyst production | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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