A hidden battle in the dirt: Soil amoebae interactions with Paracoccidioides spp

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Autor(es): dc.contributorUniversity of Brasília-
Autor(es): dc.contributorJohns Hopkins Bloomberg School of Public Health-
Autor(es): dc.contributorCatholic University of Brasília-
Autor(es): dc.contributorMilitary College-
Autor(es): dc.contributorFluminense Federal University-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorAlbuquerque, Patrícia-
Autor(es): dc.creatorNicola, André Moraes-
Autor(es): dc.creatorMagnabosco, Diogo Almeida Gomes-
Autor(es): dc.creatorda Silveira Derengowski, Lorena-
Autor(es): dc.creatorCrisóstomo, Luana Soares-
Autor(es): dc.creatorXavier, Luciano Costa Gomes-
Autor(es): dc.creatorde Oliveira Frazão, Stefânia-
Autor(es): dc.creatorGuilhelmelli, Fernanda-
Autor(es): dc.creatorDe Oliveira, Marco Antônio-
Autor(es): dc.creatordo Nascimento Dias, Jhones-
Autor(es): dc.creatorHurtado, Fabián Andrés-
Autor(es): dc.creatorde Melo Teixeira, Marcus-
Autor(es): dc.creatorGuimarães, Allan Jefferson-
Autor(es): dc.creatorPaes, Hugo Costa-
Autor(es): dc.creatorBagagli, Eduardo [UNESP]-
Autor(es): dc.creatorFelipe, Maria Sueli Soares-
Autor(es): dc.creatorCasadevall, Arturo-
Autor(es): dc.creatorSilvaPereira, Ildinete-
Data de aceite: dc.date.accessioned2022-02-22T00:27:34Z-
Data de disponibilização: dc.date.available2022-02-22T00:27:34Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2019-01-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1371/journal.pntd.0007742-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/199515-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/199515-
Descrição: dc.descriptionParacoccidioides spp. are thermodimorphic fungi that cause a neglected tropical disease (paracoccidioidomycosis) that is endemic to Latin America. These fungi inhabit the soil, where they live as saprophytes with no need for a mammalian host to complete their life cycle. Despite this, they developed sophisticated virulence attributes allowing them not only to survive in host tissues but also to cause disease. A hypothesis for selective pressures driving the emergence or maintenance of virulence of soil fungi is their interaction with soil predators such as amoebae and helminths. We evaluated the presence of environmental amoeboid predators in soil from armadillo burrows where Paracoccidioides had been previously detected and tested if the interaction of Paracoccidioides with amoebae selects for fungi with increased virulence. Nematodes, ciliates, and amoebae–all potential predators of fungi–grew in cultures from soil samples. Microscopical observation and ITS sequencing identified the amoebae as Acanthamoeba spp, Allovahlkampfia spelaea, and Vermamoeba vermiformis. These three amoebae efficiently ingested, killed and digested Paracoccidioides spp. yeast cells, as did laboratory adapted axenic Acanthamoeba castellanii. Sequential co-cultivation of Paracoccidioides with A. castellanii selected for phenotypical traits related to the survival of the fungus within a natural predator as well as in murine macrophages and in vivo (Galleria mellonella and mice). These changes in virulence were linked to the accumulation of cell wall alpha-glucans, polysaccharides that mask recognition of fungal molecular patterns by host pattern recognition receptors. Altogether, our results indicate that Paracoccidioides inhabits a complex environment with multiple amoeboid predators that can exert selective pressure to guide the evolution of virulence traits.-
Descrição: dc.descriptionFaculty of Ceilândia University of Brasília-
Descrição: dc.descriptionDepartment of Cell Biology Institute of Biological Sciences University of Brasília-
Descrição: dc.descriptionDepartment of Molecular Microbiology and Immunology Johns Hopkins Bloomberg School of Public Health-
Descrição: dc.descriptionFaculty of Medicine University of Brasília-
Descrição: dc.descriptionProgram in Genomic Sciences Catholic University of Brasília-
Descrição: dc.descriptionMilitary College-
Descrição: dc.descriptionDepartment of Microbiology and Parasitology Biomedical Institute Fluminense Federal University-
Descrição: dc.descriptionDepartment of Microbiology and Immunology Biosciences Institute UNESP-
Descrição: dc.descriptionDepartment of Microbiology and Immunology Biosciences Institute UNESP-
Idioma: dc.languageen-
Relação: dc.relationPLoS Neglected Tropical Diseases-
???dc.source???: dc.sourceScopus-
Título: dc.titleA hidden battle in the dirt: Soil amoebae interactions with Paracoccidioides spp-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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