The ATC/TTC haplotype in the Interleukin 8 gene in response to Gram-negative bacteria: A pilot study

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Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorAlfenas Federal University (UNIFAL-MG)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorPigossi, Suzane C. [UNESP]-
Autor(es): dc.creatorAnovazzi, Giovana [UNESP]-
Autor(es): dc.creatorFinoti, Livia S. [UNESP]-
Autor(es): dc.creatorde Medeiros, Marcell C. [UNESP]-
Autor(es): dc.creatorde Souza-Moreira, Tatiana Maria [UNESP]-
Autor(es): dc.creatorMayer, Marcia P.A.-
Autor(es): dc.creatorZanelli, Cleslei Fernando [UNESP]-
Autor(es): dc.creatorValentini, Sandro Roberto [UNESP]-
Autor(es): dc.creatorRossa Junior, Carlos [UNESP]-
Autor(es): dc.creatorScarel-Caminaga, Raquel M. [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:27:29Z-
Data de disponibilização: dc.date.available2022-02-22T00:27:29Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2019-10-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.archoralbio.2019.104508-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/199481-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/199481-
Descrição: dc.descriptionObjective: The aim of this study was to investigate the functionality of ATC/TTC (Hap-1) and ATT/TTC (Hap-2) Interleukin (IL) 8 gene haplotypes in the response of neutrophils to Gram-negative bacteria associated with periodontitis. Design: Neutrophils were isolated by gradient centrifugation from whole peripheral blood of systemically healthy individuals presenting the two IL8 gene haplotypes. Neutrophils were stimulated with P. gingivalis, A. actinomycetemcomitans and PMA/ionomycin. Cytokine gene expression (RT-qPCR) and migration/chemotaxis (boyden chamber assay) were compared according to the presence of Hap-1 or Hap-2 haplotypes. Protein production was also evaluted in the multiplex assay using the mixed population of leukocytes present in the whole blood from the same individuals. The influence of these two haplotypes on the IL8 promoter activity was assessed in gene-reporter experiments. Results: Hap-1 haplotype in neutrophils and leukocytes exacerbated the response to stimulation with Gram-negative bacteria, with higher levels of TNF-α (mRNA and protein), IL-1β, IL-2R and IFN-γ (protein) and with increased chemotaxis. Presence of the T allele at the rs4071 polymorphism (alias -251) was associated with increased activity of IL8 proximal promoter. Conclusions: Neutrophils and leukocytes carrying the Hap-1 haplotype (ATC/TTC) in the IL8 gene present an enhanced response to stimulation with Gram-negative bacteria associated with periodontitis. Presence of the T allele (rs4073) in the IL8 proximal promoter increases transcription activity.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionDepartment of Oral Diagnosis and Surgery School of Dentistry at Araraquara UNESP- São Paulo State University-
Descrição: dc.descriptionDepartment of Clinics and Surgery School of Dentistry Alfenas Federal University (UNIFAL-MG)-
Descrição: dc.descriptionDepartment of Biological Sciences School of Pharmaceutical Sciences UNESP- São Paulo State University-
Descrição: dc.descriptionDepartment of Microbiology Institute of Biomedical Sciences University of São Paulo-
Descrição: dc.descriptionDepartment of Morphology School of Dentistry at Araraquara UNESP- São Paulo State University-
Descrição: dc.descriptionDepartment of Oral Diagnosis and Surgery School of Dentistry at Araraquara UNESP- São Paulo State University-
Descrição: dc.descriptionDepartment of Biological Sciences School of Pharmaceutical Sciences UNESP- São Paulo State University-
Descrição: dc.descriptionDepartment of Morphology School of Dentistry at Araraquara UNESP- São Paulo State University-
Descrição: dc.descriptionCNPq: #141787/2014-7-
Idioma: dc.languageen-
Relação: dc.relationArchives of Oral Biology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectGram-negative bacteria-
Palavras-chave: dc.subjectInterleukin-8-
Palavras-chave: dc.subjectLeukocytes-
Palavras-chave: dc.subjectNeutrophils-
Palavras-chave: dc.subjectPolymorphism functionality-
Título: dc.titleThe ATC/TTC haplotype in the Interleukin 8 gene in response to Gram-negative bacteria: A pilot study-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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