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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.contributor | Federal University of Western Pará (UFOPA) | - |
Autor(es): dc.creator | Marinho-Neto, Fausto A. [UNESP] | - |
Autor(es): dc.creator | Claudiano, Gustavo S. [UNESP] | - |
Autor(es): dc.creator | Yunis-Aguinaga, Jefferson [UNESP] | - |
Autor(es): dc.creator | Cueva-Quiroz, Victor A. [UNESP] | - |
Autor(es): dc.creator | Kobashigawa, Karina K. [UNESP] | - |
Autor(es): dc.creator | Cruz, Nathan R.N. [UNESP] | - |
Autor(es): dc.creator | Moraes, Flávio R. [UNESP] | - |
Autor(es): dc.creator | Moraes, Julieta R.E. [UNESP] | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:27:23Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:27:23Z | - |
Data de envio: dc.date.issued | 2020-12-11 | - |
Data de envio: dc.date.issued | 2020-12-11 | - |
Data de envio: dc.date.issued | 2019-09-01 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1371/journal.pone.0222626 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/199442 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/199442 | - |
Descrição: dc.description | Aeromonas bacteria can cause an infection characterized by septicemia and is one of the most common pathogens in tropical fish. This disease is responsible for high morbidity and mortality rates, causing considerable losses in aquaculture. Thus, the understanding of its pathophysiology is crucial to develop control strategies of this bacterial infection in farmed fish. This study aimed to characterize early pathological aspects of acute sepsis in pacu (Piaractus mesopotamicus) experimentally infected with Aeromonas hydrophila. A total of 160 juvenile pacus were inoculated intraperitoneally with A. hydrophila (1.78 x 109 CFU/mL) and at 0 (control), 1, 3, 6, and 9 hours post-inoculation (hpi), animals were anesthetized and samples were collected for microbiological, light microscopy and transmission electron microscopy (TEM) analyzes. The results showed the occurrence of hemodynamic alterations, such as hemorrhage and congestion, which were observed mainly after 6 and 9 hpi. It was possible to re-isolate Aeromonas at all sampling times except in control group. However, just after 9 hpi it was possible to find the bacteria in all fish and tissues. Light microscopy analyses revealed a degenerative process, necrosis and vascular damage mainly at 6 and 9 hpi. According to the ultrastructural examination, areas of cellular death were identified in all examined tissues, especially at 6 and 9 hpi. However, the most severe, related to necrosis, were observed after 6 and 9 hpi. The findings suggested that this bacterium spreads in the first hpi through the fish organs, mainly affecting spleen, liver and kidney, causing irreversible lesions at the molecular level. | - |
Descrição: dc.description | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | - |
Descrição: dc.description | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | - |
Descrição: dc.description | Departament of Veterinary Pathology School of Agricultural and Veterinary Sciences São Paulo State University (UNESP) | - |
Descrição: dc.description | Institute of Biodiversity and Forests Federal University of Western Pará (UFOPA) | - |
Descrição: dc.description | Aquaculture Center of UNESP | - |
Descrição: dc.description | Departament of Veterinary Surgery and Clinic School of Agricultural and Veterinary Sciences São Paulo State University (UNESP) | - |
Descrição: dc.description | Departament of Veterinary Pathology School of Agricultural and Veterinary Sciences São Paulo State University (UNESP) | - |
Descrição: dc.description | Aquaculture Center of UNESP | - |
Descrição: dc.description | Departament of Veterinary Surgery and Clinic School of Agricultural and Veterinary Sciences São Paulo State University (UNESP) | - |
Descrição: dc.description | FAPESP: 2011/20280-2 | - |
Descrição: dc.description | FAPESP: 2014/10231-2 | - |
Descrição: dc.description | CNPq: 441054/2014-5 | - |
Idioma: dc.language | en | - |
Relação: dc.relation | PLoS ONE | - |
???dc.source???: dc.source | Scopus | - |
Título: dc.title | Morphological, microbiological and ultrastructural aspects of sepsis by Aeromonas hydrophila in Piaractus mesopotamicus | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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