Local and systemic immune mediators of Morada Nova lambs with divergent Haemonchus contortus resistance phenotypes

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)-
Autor(es): dc.contributorCentro Universitário Central Paulista-
Autor(es): dc.creatorBarbosa Toscano, João Henrique [UNESP]-
Autor(es): dc.creatorOkino, Cintia Hiromi-
Autor(es): dc.creatorBarbosa dos Santos, Isabella [UNESP]-
Autor(es): dc.creatorGiraldelo, Luciana Aparecida-
Autor(es): dc.creatorBorsch von Haehling, Marei [UNESP]-
Autor(es): dc.creatorNovita Esteves, Sérgio-
Autor(es): dc.creatorBassetto, Cesar Cristiano [UNESP]-
Autor(es): dc.creatorTalamini do Amarante, Alessandro Francisco [UNESP]-
Autor(es): dc.creatorde Souza Chagas, Ana Carolina-
Data de aceite: dc.date.accessioned2022-02-22T00:27:15Z-
Data de disponibilização: dc.date.available2022-02-22T00:27:15Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2019-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1111/pim.12790-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/199397-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/199397-
Descrição: dc.descriptionAims: Local and systemic immune mediators of Morada Nova lambs with divergent Haemonchus contortus resistance phenotypes were evaluated. Methods and Results: Lambs were ranked through faecal egg counts (FEC) after two parasitic challenges with 4,000 H.contortus L3. After the second challenge, the lambs underwent a third artificial infection and were euthanized 7 days later. Immune-related genes were quantified locally in abomasal mucosa and lymph nodes (CD4, IFNγ, IL4, IL5, IL13, IL2RA and MS4A2) and systemically in the whole blood (IL4 and IL13). Anti-H. contortus IgG and IgA antibodies and eosinophils and mast cells counts were also investigated. Resistant animals presented higher systemic IgG and IgA titres, both negatively correlated with FEC. Susceptible animals had higher blood levels of IL4 transcripts. At the local level, resistant lambs had higher eosinophils counts and superior MS4A2 levels in abomasal fundic mucosa, besides higher IgA levels in abomasal mucus, while susceptible lamb had superior IL4 expression in abomasal lymph nodes. Conclusion: These data indicate that resistant lambs had an immune response mediated by antibody-mediated cytotoxicity. Also, the systemic humoral profile, particularly IgA isotype, seems to be a good resistance marker for Morada Nova sheep, as we found differences between groups even when FEC did not differ.-
Descrição: dc.descriptionFaculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (UNESP)-
Descrição: dc.descriptionEmbrapa Pecuária Sudeste-
Descrição: dc.descriptionCentro Universitário Central Paulista-
Descrição: dc.descriptionInstituto de Biociências Universidade Estadual Paulista (UNESP)-
Descrição: dc.descriptionFaculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (UNESP)-
Descrição: dc.descriptionInstituto de Biociências Universidade Estadual Paulista (UNESP)-
Idioma: dc.languageen-
Relação: dc.relationParasite Immunology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectELISA-
Palavras-chave: dc.subjecteosinophils-
Palavras-chave: dc.subjectgene expression.-
Palavras-chave: dc.subjectHaemonchus contortus-
Palavras-chave: dc.subjecthost resistance-
Palavras-chave: dc.subjectMorada Nova sheep breed-
Título: dc.titleLocal and systemic immune mediators of Morada Nova lambs with divergent Haemonchus contortus resistance phenotypes-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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