Reversible and irreversible fluorescence activity of the Enhanced Green Fluorescent Protein in pH: Insights for the development of pH-biosensors

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorRMIT University-
Autor(es): dc.contributorAustralian Synchrotron-
Autor(es): dc.contributorCIEPQPF-
Autor(es): dc.creatordos Santos, Nathalia Vieira [UNESP]-
Autor(es): dc.creatorSaponi, Carolina Falaschi [UNESP]-
Autor(es): dc.creatorRyan, Timothy M.-
Autor(es): dc.creatorPrimo, Fernando L. [UNESP]-
Autor(es): dc.creatorGreaves, Tamar L.-
Autor(es): dc.creatorPereira, Jorge F.B. [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:27:07Z-
Data de disponibilização: dc.date.available2022-02-22T00:27:07Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.ijbiomac.2020.08.224-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/199340-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/199340-
Descrição: dc.descriptionEnhanced Green Fluorescent Protein (EGFP) is a biomolecule with intense and natural fluorescence, with biological and medical applications. Although widely used as a biomarker in research, its application as a biosensor is limited by the lack of in-depth knowledge regarding its structure and behavior in adverse conditions. This study is focused on addressing this need by evaluating EGFP activity and structure at different pH using three-dimensional fluorescence, circular dichroism and small-angle X-ray scattering. The focus was on the reversibility of the process to gain insights for the development of biocompatible pH-biosensors. EGFP was highly stable at alkaline pH and quenched from neutral-to-acidic pH. Above pH 6.0, the fluorescence loss was almost completely reversible on return to neutral pH, but only partially reversible from pH 5.0 to 2.0. This work updates the knowledge regarding EGFP behavior in pH by accounting for the recent data on its structure. Hence, it is evident that EGFP presents the required properties for use as natural, biocompatible and environmentally friendly neutral to acidic pH-biosensors.-
Descrição: dc.descriptionAustralian Nuclear Science and Technology Organisation-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Engineering of Bioprocesses and Biotechnology School of Pharmaceutical Sciences São Paulo State University (UNESP), Rodovia Araraquara-Jaú/Km 01-
Descrição: dc.descriptionSchool of Science College of Science Engineering and Health RMIT University, 124 La Trobe Street-
Descrição: dc.descriptionAustralian Synchrotron, 800 Blackburn Road-
Descrição: dc.descriptionUniv Coimbra CIEPQPF Department of Chemical Engineering, Rua Sílvio Lima, Pólo II – Pinhal de Marrocos-
Descrição: dc.descriptionDepartment of Engineering of Bioprocesses and Biotechnology School of Pharmaceutical Sciences São Paulo State University (UNESP), Rodovia Araraquara-Jaú/Km 01-
Descrição: dc.descriptionFAPESP: 18/06576-5-
Descrição: dc.descriptionFAPESP: 2014/16424-7-
Descrição: dc.descriptionFAPESP: 2014/19793-3-
Descrição: dc.descriptionFAPESP: 2016/07529-5-
Descrição: dc.descriptionFAPESP: 2018/01858-2-
Descrição: dc.descriptionFAPESP: 2018/20833-0-
Descrição: dc.descriptionFAPESP: 2018/50009-8-
Formato: dc.format3474-3484-
Idioma: dc.languageen-
Relação: dc.relationInternational Journal of Biological Macromolecules-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBiosensor-
Palavras-chave: dc.subjectCircular dichroism (CD)-
Palavras-chave: dc.subjectFluorescence-
Palavras-chave: dc.subjectGreen Fluorescent Protein-
Palavras-chave: dc.subjectIntrinsic fluorescence of proteins-
Palavras-chave: dc.subjectProtein stability-
Palavras-chave: dc.subjectSmall-angle X-ray scattering (SAXS)-
Título: dc.titleReversible and irreversible fluorescence activity of the Enhanced Green Fluorescent Protein in pH: Insights for the development of pH-biosensors-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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