Biocompatibility, induction of mineralization and antimicrobial activity of experimental intracanal pastes based on glass and glass-ceramic materials

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade Federal de Minas Gerais (UFMG)-
Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.creatorde Araújo Lopes, J. M. [UNESP]-
Autor(es): dc.creatorBenetti, F. [UNESP]-
Autor(es): dc.creatorRezende, G. C. [UNESP]-
Autor(es): dc.creatorSouza, M. T.-
Autor(es): dc.creatorConti, L. C. [UNESP]-
Autor(es): dc.creatorErvolino, E. [UNESP]-
Autor(es): dc.creatorJacinto, R. C. [UNESP]-
Autor(es): dc.creatorZanotto, E. D.-
Autor(es): dc.creatorCintra, L. T.A. [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:27:03Z-
Data de disponibilização: dc.date.available2022-02-22T00:27:03Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2019-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1111/iej.13382-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/199323-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/199323-
Descrição: dc.descriptionAim: To evaluate the biocompatibility, induction of mineralization and antimicrobial activity of experimental intracanal pastes based on two glass and glass-ceramic materials. Calcium hydroxide (Ca(OH)2) paste was used as the positive control. Methodology: The glass-ceramic powder [two-phased Biosilicate (BS-2P)] and F18 bioactive glass were mixed with distilled water (ratio 2 : 1), inserted in polyethylene tubes and implanted in the subcutaneous tissues of 16 rats. Empty tubes were used as negative control. After 7 and 30 days (n = 8), the rats were euthanized for haematoxylin–eosin, von Kossa, polarized light and osteopontin (OPN) immunolabeling analysis. Direct contact tests using a suspension of each paste were performed with Enterococcus faecalis planktonic cells to evaluate antimicrobial activity (24 h of contact), in a pilot study. The number of CFU mL−1 was calculated for each group. The antimicrobial analysis data were submitted to one-way anova and Tukey tests, whilst biocompatibility and immunohistochemical data were submitted to the Kruskal–Wallis and Dunn tests (P < 0.05). Results: Most specimens of the control, BS-2P and Ca(OH)2 groups were associated with moderate inflammation seven days following implantation, whilst F18 was associated with moderate to severe inflammation, without differences amongst the groups (P > 0.05). At 30 days, most specimens of control, F18 and BS-2P groups had mild inflammation, whilst Ca(OH)2 had mild to moderate inflammation; however, no differences were determined amongst the groups (P > 0.05). The fibrous capsule was thick at 7 days, becoming thin at 30 days. All pastes induced von Kossa-positive structures and were birefringent to polarized light. At seven days, the BS-2P group had significantly more OPN immunolabeling compared to the control and Ca(OH)2 groups (P < 0.05). At 30 days, the F18 group had significantly more OPN immunolabeling compared to the control and Ca(OH)2 groups (P < 0.05). All pastes reduced the total number of E. faecalis; however, the reduction was only significant when comparing BS-2P and Ca(OH)2 groups to the control (P < 0.05). Only calcium hydroxide eliminated E. faecalis. Conclusions: Experimental BS-2P and F18 pastes were biocompatible, stimulated biomineralization and induced significant OPN immunolabeling compared to Ca(OH)2. Only the BS-2P paste demonstrated antimicrobial activity comparable to Ca(OH)2.-
Descrição: dc.descriptionEndodontics Section Department of Preventive and Restorative Dentistry School of Dentistry São Paulo State University (Unesp)-
Descrição: dc.descriptionEndodontic Section Department of Restorative Dentistry School of Dentistry Universidade Federal de Minas Gerais-
Descrição: dc.descriptionVitreous Materials Laboratory (LaMaV) Department of Materials Engineering Federal University of São Carlos (UFSCar)-
Descrição: dc.descriptionDepartment of Basic Science School of Dentistry São Paulo State University (Unesp)-
Descrição: dc.descriptionEndodontics Section Department of Preventive and Restorative Dentistry School of Dentistry São Paulo State University (Unesp)-
Descrição: dc.descriptionDepartment of Basic Science School of Dentistry São Paulo State University (Unesp)-
Idioma: dc.languageen-
Relação: dc.relationInternational Endodontic Journal-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectbioactive glass-
Palavras-chave: dc.subjectbioceramics-
Palavras-chave: dc.subjectbiocompatibility-
Palavras-chave: dc.subjectbiomineralization-
Palavras-chave: dc.subjectosteopontin-
Título: dc.titleBiocompatibility, induction of mineralization and antimicrobial activity of experimental intracanal pastes based on glass and glass-ceramic materials-
Tipo de arquivo: dc.typelivro digital-
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