Characterization of pomegranate peel extracts obtained using different solvents and their effects on cell cycle and apoptosis in leukemia cells

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorMackenzie Presbyterian University-
Autor(es): dc.creatorTamborlin, Leticia [UNESP]-
Autor(es): dc.creatorSumere, Beatriz Rocchetti-
Autor(es): dc.creatorde Souza, Mariana Corrêa-
Autor(es): dc.creatorPestana, Nathalie Fortes-
Autor(es): dc.creatorAguiar, Ana Carolina-
Autor(es): dc.creatorEberlin, Marcos Nogueira-
Autor(es): dc.creatorSimabuco, Fernando Moreira-
Autor(es): dc.creatorRostagno, Maurício Ariel-
Autor(es): dc.creatorLuchessi, Augusto Ducati [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:27:02Z-
Data de disponibilização: dc.date.available2022-02-22T00:27:02Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2019-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1002/fsn3.1831-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/199312-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/199312-
Descrição: dc.descriptionPomegranate (Punica granatum L.) has been used in traditional herbal medicine by several cultures as an anti-inflammatory, antioxidant, antihyperglycemic, and for treatment and prevention of cancer and other diseases. Different parts of the fruit, extraction methods, and solvents can define the chemical profile of the obtained extracts and their biological activities. This study aimed to characterize the chemical profile of peel extracts collected using different extraction solvents and their biological effects on the cell cycle and apoptosis of THP-1 leukemic cells. Aqueous extract presented the highest content of punicalagins (α pun = 562.26 ± 47.14 mg/L and β pun = 1,251.13 ± 22.21 mg/L) and the lowest content of ellagic acid (66.38 ± 0.21 mg/L), and it promoted a significant impairment of the cell cycle S phase. In fact, punicalagin-enriched fraction, but not an ellagic acid-enriched fraction, caused an S phase cell cycle arrest. All extracts increased the number of apoptotic cells. Punicalagin-enriched fraction increased the percentage of cells with fragmented DNA, which was intensified by ellagic acid combination. The treatment combining punicalagin and ellagic acid fractions increased the apoptotic cleaved PARP1 protein and reduced the activation of the growth-related mTOR pathway. Thus, these results evidence that solvent choice is critical for the phenolic compounds profile of pomegranate peel extracts and their biological activities.-
Descrição: dc.descriptionLaboratory of Biotechnology (BioTech) School of Applied Sciences (FCA) University of Campinas (UNICAMP)-
Descrição: dc.descriptionInstitute of Biosciences (IB) São Paulo State University (UNESP)-
Descrição: dc.descriptionMultidisciplinary Laboratory of Food and Health (LabMAS) School of Applied Sciences (FCA) University of Campinas (UNICAMP)-
Descrição: dc.descriptionThoMSon Mass Spectrometry Laboratory Institute of Chemistry (IQ) University of Campinas (UNICAMP)-
Descrição: dc.descriptionMackMass Laboratory School of Engineering (PPGEMN) Mackenzie Presbyterian University-
Descrição: dc.descriptionInstitute of Biosciences (IB) São Paulo State University (UNESP)-
Idioma: dc.languageen-
Relação: dc.relationFood Science and Nutrition-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectapoptosis-
Palavras-chave: dc.subjectbiological activity-
Palavras-chave: dc.subjectcell cycle-
Palavras-chave: dc.subjectleukemia-
Palavras-chave: dc.subjectpomegranate peel extracts-
Palavras-chave: dc.subjectpunicalagin-
Título: dc.titleCharacterization of pomegranate peel extracts obtained using different solvents and their effects on cell cycle and apoptosis in leukemia cells-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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