Production of β-galactosidase by Trichoderma sp. through solid-state fermentation targeting the recovery of galactooligosaccharides from whey cheese

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorde Jesus, L. F.M.C. [UNESP]-
Autor(es): dc.creatorGuimarães, L. H.S.-
Data de aceite: dc.date.accessioned2022-02-22T00:26:53Z-
Data de disponibilização: dc.date.available2022-02-22T00:26:53Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2019-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1111/jam.14805-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/199258-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/199258-
Descrição: dc.descriptionAims: Optimization of β-galactosidase production by Trichoderma sp. under solid-state fermentation using wheat bran as solid substrate through an experimental design and its application targeting the recovery of galactooligosaccharides (GOS) from whey cheese. Methods and Results: The β-galactosidase production by Trichoderma sp. increased 2·3-fold (2·67 U g−1 of substrate) culturing the fungus at 30°C for 187 h, at an inoculum of 105 spores per ml, and a 1 : 1·65 (w/v) ratio of wheat bran to tap water. The best enzyme activity was obtained at 55°C and pH 4·5. The catalytic activity was maintained for up to 180 min incubating at 35–45°C, and above 50% at acidic or alkaline pH for up to 24 h. It also presented resistance to chemical compounds. β-galactosidase catalysed the hydrolysis of the lactose and the transgalactosylation reaction leading to the production of GOS. Conclusion: Trichoderma sp. produced β-galactosidase with transgalactosylation activity that may be used to recover GOS, products with high added value, from whey cheese. Significance and Impact of the Study: β-galactosidases are used in different industrial sectors. Therefore, the Trichoderma β-galactosidase is a promising alternative for the production of GOS as prebiotic from the dairy effluents, contributing to the reduction in the environmental impact.-
Descrição: dc.descriptionInstituto de Química de Araraquara-UNESP-
Descrição: dc.descriptionFaculdade de Filosofia Ciências e Letras de Ribeirão Preto USP-
Descrição: dc.descriptionInstituto de Química de Araraquara-UNESP-
Idioma: dc.languageen-
Relação: dc.relationJournal of Applied Microbiology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectdairy effluent-
Palavras-chave: dc.subjectgalactooligosaccharides-
Palavras-chave: dc.subjectgalactosidase-
Palavras-chave: dc.subjectlactase-
Palavras-chave: dc.subjectsolid-state fermentation-
Palavras-chave: dc.subjectTrichoderma-
Título: dc.titleProduction of β-galactosidase by Trichoderma sp. through solid-state fermentation targeting the recovery of galactooligosaccharides from whey cheese-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.