Enhancement of Clostridium butyricum hydrogen production by iron and nickel nanoparticles: Effects on hydA expression

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.creatorMoura, A. G.L.-
Autor(es): dc.creatorRabelo, C. A.B.S.-
Autor(es): dc.creatorOkino, C. H.-
Autor(es): dc.creatorMaintinguer, S. I. [UNESP]-
Autor(es): dc.creatorSilva, E. L.-
Autor(es): dc.creatorVaresche, M. B.A.-
Data de aceite: dc.date.accessioned2022-02-22T00:26:50Z-
Data de disponibilização: dc.date.available2022-02-22T00:26:50Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2019-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.ijhydene.2020.07.161-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/199239-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/199239-
Descrição: dc.descriptionIn this study, an optimized medium for Clostridium butyricum (CCT7470) was obtained from a Plackett-Burman assay. It was evidenced that glucose (5 g/L), starch (1 g/L) and three nitrogen and vitamin sources (5 g meat extract/L; 5 g peptone/L and 1.5 g yeast extract/L) improved hydrogen (H2) production. The addition of nanoparticles (NPs) improved the fermentation process, and maximum H2 production (434 ± 13 mL) was achieved with 30 mg Fe0/L and 20 mg Ni0/L. The metabolic pathway shifted from acetic and butyric to valeric acid production with the addition of NPs. In reactors with NPs, a longer permanence of hydrogenase was observed with hydA quantification by real-time polymerase chain reaction (qPCR). However, the expression of new enzymes, assessed by reverse transcription followed by qPCR (RT-qPCR), was higher in control assay than that in the NPs one.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionLaboratory of Biological Processes Department of Hydraulics and Sanitation Engineering School of São Carlos – University of São Paulo (EESC – USP)-
Descrição: dc.descriptionEmbrapa Southeast Livestock, Washington Luiz Km 234, Farm Canchim-
Descrição: dc.descriptionCentral Laboratory Bioenergy Research Institute São Paulo State University (IPBEN-UNESP)-
Descrição: dc.descriptionDepartment of Chemical Engineering Federal University of São Carlos-
Descrição: dc.descriptionCentral Laboratory Bioenergy Research Institute São Paulo State University (IPBEN-UNESP)-
Idioma: dc.languageen-
Relação: dc.relationInternational Journal of Hydrogen Energy-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectClostridium butyricum-
Palavras-chave: dc.subjectDark fermentation-
Palavras-chave: dc.subjectIron nanoparticles-
Palavras-chave: dc.subjectNickel nanoparticles-
Palavras-chave: dc.subjectResponse surface methodology-
Título: dc.titleEnhancement of Clostridium butyricum hydrogen production by iron and nickel nanoparticles: Effects on hydA expression-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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