Circulating miR-16-5p, miR-92a-3p, and miR-451a in plasma from lung cancer patients: Potential application in early detection and a regulatory role in tumorigenesis pathways

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Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorInstituto de Investigación-
Autor(es): dc.contributorUniversity Health Network-
Autor(es): dc.contributorUniversity of Toronto-
Autor(es): dc.contributorPrincess Margaret Cancer Centre-
Autor(es): dc.creatorReis, Patricia P. [UNESP]-
Autor(es): dc.creatorDrigo, Sandra A. [UNESP]-
Autor(es): dc.creatorCarvalho, Robson F. [UNESP]-
Autor(es): dc.creatorLapa, Rainer Marco Lopez-
Autor(es): dc.creatorFelix, Tainara F. [UNESP]-
Autor(es): dc.creatorPatel, Devalben-
Autor(es): dc.creatorCheng, Dangxiao-
Autor(es): dc.creatorPintilie, Melania-
Autor(es): dc.creatorLiu, Geoffrey-
Autor(es): dc.creatorTsao, Ming-Sound-
Data de aceite: dc.date.accessioned2022-02-22T00:26:41Z-
Data de disponibilização: dc.date.available2022-02-22T00:26:41Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-08-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/cancers12082071-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/199184-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/199184-
Descrição: dc.descriptionBackground: Micro(mi)RNAs, potent gene expression regulators associated with tumorigenesis, are stable, abundant circulating molecules, and detectable in plasma. Thus, miRNAs could potentially be useful in early lung cancer detection. We aimed to identify circulating miRNA signatures in plasma from patients with lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC), and to verify whether miRNAs regulate lung oncogenesis pathways. Methods: RNA isolated from 139 plasma samples (40 LUAD, 38 LUSC; 61 healthy/non-diseased individuals) were divided into discovery (38 patients; 21 controls for expression quantification using an 800-miRNA panel; Nanostring nCounter®) and validation (40 patients; 40 controls; TaqMan® RT-qPCR) cohorts. Elastic net, Maximizing-R-Square Analysis (MARSA), and C-Statistics were applied for miRNA signature identification. Results: When compared to healthy individuals, 580 of 606 deregulated miRNAs in LUAD and 221 of 226 deregulated miRNAs in LUSC had significantly increased levels. Among the 10 most significantly overexpressed miRNAs, 6 were common to patients with LUAD and LUSC. Further analysis identified three signatures composed of 12 miRNAs. Signatures included miRNAs commonly overexpressed in patient plasma. Enriched pathways included target genes modulated by three miRNAs in the C-Statistics signature: miR-16-5p, miR-92a-3p, and miR-451a. Conclusions: The 3-miRNA signature (miR-16-5p, miR-92a-3p, miR-451a) had high specificity (100%) and sensitivity (84%) to predict cancer (LUAD and LUSC). These miRNAs are predicted to modulate genes and pathways with known roles in lung tumorigenesis, including EGFR, K-RAS, and PI3K/AKT signaling, suggesting that the 3-miRNA signature is biologically relevant in adenocarcinoma and squamous cell carcinoma of the lung.-
Descrição: dc.descriptionPrincess Margaret Cancer Foundation-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionHealth Research Foundation-
Descrição: dc.descriptionFaculty of Medicine São Paulo State University UNESP-
Descrição: dc.descriptionExperimental Research Unity São Paulo State University UNESP-
Descrição: dc.descriptionDepartment of Structural and Functional Biology Institute of Biosciences São Paulo State University UNESP-
Descrição: dc.descriptionUniversidad Católica Los Ángeles de Chimbote Instituto de Investigación-
Descrição: dc.descriptionPrincess Margaret Cancer Centre University Health Network-
Descrição: dc.descriptionDepartment of Medical Biophysics University of Toronto-
Descrição: dc.descriptionDepartment of Medical Oncology and Hematology Princess Margaret Cancer Centre-
Descrição: dc.descriptionDepartment of Laboratory Medicine and Pathobiology University of Toronto-
Descrição: dc.descriptionLaboratory Medicine Program University Health Network-
Descrição: dc.descriptionFaculty of Medicine São Paulo State University UNESP-
Descrição: dc.descriptionExperimental Research Unity São Paulo State University UNESP-
Descrição: dc.descriptionDepartment of Structural and Functional Biology Institute of Biosciences São Paulo State University UNESP-
Descrição: dc.descriptionFAPESP: # 2016/09021-9-
Descrição: dc.descriptionHealth Research Foundation: FDN-148395-
Formato: dc.format1-16-
Idioma: dc.languageen-
Relação: dc.relationCancers-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBiomarkers-
Palavras-chave: dc.subjectLiquid biopsy-
Palavras-chave: dc.subjectLung adenocarcinoma-
Palavras-chave: dc.subjectLung cancer-
Palavras-chave: dc.subjectLung squamous cell carcinoma-
Palavras-chave: dc.subjectLung tumorigenesis-
Palavras-chave: dc.subjectMicroRNAs-
Palavras-chave: dc.subjectPathways-
Palavras-chave: dc.subjectPlasma-
Título: dc.titleCirculating miR-16-5p, miR-92a-3p, and miR-451a in plasma from lung cancer patients: Potential application in early detection and a regulatory role in tumorigenesis pathways-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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