Chitosan ameliorates candida auris virulence in a galleria mellonella infection model

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Autor(es): dc.contributorUniversity of Glasgow-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorGlasgow Caledonian University-
Autor(es): dc.contributorGlasgow Dental School-
Autor(es): dc.contributorUniversity of Western São Paulo (UNOESTE)-
Autor(es): dc.creatorArias, Laís Salomão [UNESP]-
Autor(es): dc.creatorButche, Mark C.-
Autor(es): dc.creatorShort, Bryn-
Autor(es): dc.creatorMcKloud, Emily-
Autor(es): dc.creatorDelaney, Chris-
Autor(es): dc.creatorKean, Ryan-
Autor(es): dc.creatorMonteiro, Douglas Roberto [UNESP]-
Autor(es): dc.creatorWilliams, Craig-
Autor(es): dc.creatorRamage, Gordon-
Autor(es): dc.creatorBrow, Jason L.-
Data de aceite: dc.date.accessioned2022-02-22T00:26:38Z-
Data de disponibilização: dc.date.available2022-02-22T00:26:38Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-08-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1128/AAC.00476-20-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/199161-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/199161-
Descrição: dc.descriptionCandida auris has emerged as a multidrug-resistant nosocomial pathogen over the last decade. Outbreaks of the organism in health care facilities have resulted in life-threatening invasive candidiasis in over 40 countries worldwide. Resistance by C. auris to conventional antifungal drugs such as fluconazole and amphotericin B means that alternative therapeutics must be explored. As such, this study served to investigate the efficacy of a naturally derived polysaccharide called chitosan against aggregative (Agg) and nonaggregative (non-Agg) isolates of C. auris in vitro and in vivo. In vitro results indicated that chitosan was effective against planktonic and sessile forms of Agg and non-Agg C. auris. In a Galleria mellonella model to assess C. auris virulence, chitosan treatment was shown to ameliorate killing effects of both C. auris phenotypes (NCPF 8973 and NCPF 8978, respectively) in vivo. Specifically, chitosan reduced the fungal load and increased survival rates of infected Galleria, while treatment alone was nontoxic to the larvae. Finally, chitosan treatment appeared to induce a stress-like gene expression response in NCPF 8973 in the larvae likely arising from a protective response by the organism to resist antifungal activity of the compound. Taken together, results from this study demonstrate that naturally derived compounds such as chitosan may be useful alternatives to conventional antifungals against C. auris.-
Descrição: dc.descriptionOral Sciences Research Group Glasgow Dental School School of Medicine College of Medical Veterinary and Life Sciences University of Glasgow-
Descrição: dc.descriptionSão Paulo State University (Unesp) School of Dentistry Department of Preventive and Restorative Dentistry-
Descrição: dc.descriptionDepartment of Biological and Biomedical Sciences School of Health and Life Sciences Glasgow Caledonian University-
Descrição: dc.descriptionGlasgow Biofilm Research Network Glasgow Dental School-
Descrição: dc.descriptionGraduate Program in Dentistry University of Western São Paulo (UNOESTE)-
Descrição: dc.descriptionSão Paulo State University (Unesp) School of Dentistry Department of Preventive and Restorative Dentistry-
Idioma: dc.languageen-
Relação: dc.relationAntimicrobial Agents and Chemotherapy-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAggregates-
Palavras-chave: dc.subjectCandida auris-
Palavras-chave: dc.subjectChitosan-
Palavras-chave: dc.subjectGalleria mellonella-
Palavras-chave: dc.subjectTherapeutics-
Título: dc.titleChitosan ameliorates candida auris virulence in a galleria mellonella infection model-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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