Candida albicans Mrv8, is involved in epithelial damage and biofilm formation

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorHans-Knoell-Institute-
Autor(es): dc.contributorInstitute of Medical Sciences-
Autor(es): dc.contributorFriedrich Schiller University-
Autor(es): dc.contributorUniversity of Exeter-
Autor(es): dc.creatorCosta, Anna Carolina Borges Pereira [UNESP]-
Autor(es): dc.creatorBack-Brito, Graziella Nuernberg [UNESP]-
Autor(es): dc.creatorMayer, François L.-
Autor(es): dc.creatorHube, Bernhard-
Autor(es): dc.creatorWilson, Duncan-
Data de aceite: dc.date.accessioned2022-02-22T00:26:31Z-
Data de disponibilização: dc.date.available2022-02-22T00:26:31Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-08-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1093/femsyr/foaa033-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/199127-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/199127-
Descrição: dc.descriptionCandida albicans is the most common human fungal pathogen that can cause superficial and deep-seated infections in susceptible individuals. Despite its medical importance, the vast majority of C. albicans genes remain of unknown function. Here, we report a role for the lineage-specific gene, MRV8, in host pathogen interactions, mycelial microcolony maturation and biofilm formation. In silico analysis indicated that MRV8 encodes a four-pass transmembrane protein unique to the closely related pathogens C. albicans and Candida dubliniensis. Deletion of MRV8 did not affect C. albicans adherence to, or initial invasion into human oral epithelia, but inhibited mycelial development and strongly reduced epithelial damage. mrv8Δ/Δ cells exhibited a media-dependent defect in biofilm formation and mutant biofilm metabolic activity was enhanced by cyclosporin A. mrv8Δ/Δ biofilms were more tolerant to treatment with caspofungin, but not to fluconazole or amphotericin B. Co-stimulation with calcium chloride and calcofluor white rescued biofilm growth in the presence of caspofungin, and this rescue-effect was Mrv8-dependent. Together, our data demonstrate an important role for a lineage-specific gene (MRV8) in C. albicans biofilm formation, drug tolerance and host-pathogen interactions.-
Descrição: dc.descriptionJena School for Microbial Communication, Friedrich-Schiller-Universität Jena-
Descrição: dc.descriptionScuola Internazionale Superiore di Studi Avanzati-
Descrição: dc.descriptionWellcome Trust-
Descrição: dc.descriptionDeutscher Akademischer Austauschdienst-
Descrição: dc.descriptionCenter for Sepsis Control and Care-
Descrição: dc.descriptionUniversity of Exeter-
Descrição: dc.descriptionSeventh Framework Programme-
Descrição: dc.descriptionDepartment of Biosciences and Oral Diagnosis São Paulo State University (Unesp) Institute of Science and Technology (ICT)-
Descrição: dc.descriptionDepartment of Microbial Pathogenicity Mechanisms Hans-Knoell-Institute-
Descrição: dc.descriptionAberdeen Fungal Group School of Medicine Medical Sciences and Nutrition University of Aberdeen Institute of Medical Sciences-
Descrição: dc.descriptionFriedrich Schiller University-
Descrição: dc.descriptionMedical Research Council Centre for Medical Mycology School of Biosciences University of Exeter, Stocker Rd-
Descrição: dc.descriptionDepartment of Biosciences and Oral Diagnosis São Paulo State University (Unesp) Institute of Science and Technology (ICT)-
Descrição: dc.descriptionWellcome Trust: 09 7377/Z/11/Z-
Descrição: dc.descriptionDeutscher Akademischer Austauschdienst: 12 417/12-2-
Descrição: dc.descriptionWellcome Trust: 214317/Z/18/Z-
Descrição: dc.descriptionCenter for Sepsis Control and Care: BMBF 01EO1002-
Descrição: dc.descriptionUniversity of Exeter: MR/N006364/2-
Descrição: dc.descriptionSeventh Framework Programme: PIEF-GA-2008-219 406-
Idioma: dc.languageen-
Relação: dc.relationFEMS Yeast Research-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBiofilm-
Palavras-chave: dc.subjectCalcium homeostasis-
Palavras-chave: dc.subjectCandida albicans-
Palavras-chave: dc.subjectCaspofungin-
Palavras-chave: dc.subjectHost-pathogen interaction-
Palavras-chave: dc.subjectMarvel family-
Título: dc.titleCandida albicans Mrv8, is involved in epithelial damage and biofilm formation-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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