Modulating chitosan-PLGA nanoparticle properties to design a co-delivery platform for glioblastoma therapy intended for nose-to-brain route

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversity of Minho-
Autor(es): dc.contributorICVS/3B’s-PT Government Associate Laboratory-
Autor(es): dc.contributorBarretos Cancer Hospital-
Autor(es): dc.creatorFerreira, Natália N. [UNESP]-
Autor(es): dc.creatorGranja, Sara-
Autor(es): dc.creatorBoni, Fernanda I. [UNESP]-
Autor(es): dc.creatorPrezotti, Fabíola G. [UNESP]-
Autor(es): dc.creatorFerreira, Leonardo M. B. [UNESP]-
Autor(es): dc.creatorCury, Beatriz S. F. [UNESP]-
Autor(es): dc.creatorReis, Rui M.-
Autor(es): dc.creatorBaltazar, Fátima-
Autor(es): dc.creatorGremião, Maria Palmira D. [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:26:29Z-
Data de disponibilização: dc.date.available2022-02-22T00:26:29Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2019-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s13346-020-00824-2-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/199114-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/199114-
Descrição: dc.descriptionNose-to-brain delivery is a promising approach to target drugs into the brain, avoiding the blood-brain barrier and other drawbacks related to systemic absorption, and enabling an effective and safer treatment of diseases such as glioblastoma (GBM). Innovative materials and technologies that improve residence time in the nasal cavity and modulate biological interactions represent a great advance in this field. Mucoadhesive nanoparticles (NPs) based on poly(lactic-co-glycolic acid) (PLGA) and oligomeric chitosan (OCS) were designed as a rational strategy and potential platform to co-deliver alpha-cyano-4-hydroxycinnamic acid (CHC) and the monoclonal antibody cetuximab (CTX) into the brain, by nasal administration. The influence of formulation and process variables (O/Aq volume ratio, Pluronic concentration, PLGA concentration, and sonication time) on the properties of CHC-loaded NPs (size, zeta potential, PDI and entrapment efficiency) was investigated by a two-level full factorial design (24). Round, stable nano-sized particles (213–875 nm) with high positive surface charge (+ 33.2 to + 58.9 mV) and entrapment efficiency (75.69 to 93.23%) were produced by the emulsification/evaporation technique. Optimal process conditions were rationally selected based on a set of critical NP attributes (258 nm, + 37 mV, and 88% EE) for further conjugation with CTX. The high cytotoxicity of CHC-loaded NPs and conjugated NPs was evidenced for different glioma cell lines (U251 and SW1088). A chicken chorioallantoic membrane assay highlighted the expressive antiangiogenic activity of CHC-loaded NPs, which was enhanced for conjugated NPs. The findings of this work demonstrated the potential of this nanostructured polymeric platform to become a novel therapeutic alternative for GBM treatment. [Figure not available: see fulltext.].-
Descrição: dc.descriptionSchool of Pharmaceutical Science São Paulo State University UNESP, Rodovia Araraquara/Jaú Km 01-
Descrição: dc.descriptionLife and Health Sciences Research Institute (ICVS) School of Medicine University of Minho-
Descrição: dc.descriptionICVS/3B’s-PT Government Associate Laboratory-
Descrição: dc.descriptionMolecular Oncology Research Center Barretos Cancer Hospital-
Descrição: dc.descriptionSchool of Pharmaceutical Science São Paulo State University UNESP, Rodovia Araraquara/Jaú Km 01-
Idioma: dc.languageen-
Relação: dc.relationDrug Delivery and Translational Research-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCetuximab-
Palavras-chave: dc.subjectChicken chorioallantoic membrane assay-
Palavras-chave: dc.subjectGlioblastoma-
Palavras-chave: dc.subjectNanotechnology-
Palavras-chave: dc.subjectSW1088 glioma cell line-
Palavras-chave: dc.subjectU251 glioma cell line-
Palavras-chave: dc.subjectα-Cyano-4-hydroxycinnamic acid-
Título: dc.titleModulating chitosan-PLGA nanoparticle properties to design a co-delivery platform for glioblastoma therapy intended for nose-to-brain route-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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