Development and characterization of nuclear microsatellite markers for Eremanthus erythropappus and their transferability across related species

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade Federal de Lavras (UFLA)-
Autor(es): dc.contributorUniversidade Federal de Minas Gerais (UFMG)-
Autor(es): dc.creatorRocha, Lucas Fernandes [UNESP]-
Autor(es): dc.creatorPaula, Natália Ribeiro-
Autor(es): dc.creatorNazareno, Alison Gonçalves-
Autor(es): dc.creatorDe Carvalho, Dulcinéia-
Data de aceite: dc.date.accessioned2022-02-22T00:26:26Z-
Data de disponibilização: dc.date.available2022-02-22T00:26:26Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-07-07-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1186/s40659-020-00298-z-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/199089-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/199089-
Descrição: dc.descriptionBackground: We developed simple sequence repeats (SSR) for Eremanthus erythropappus (DC.) MacLeish, an endangered tree species endemic to the Brazilian Savanna and Atlantic Forest biomes, and tested their transferability to two closely related Eremanthus species. Results: Using a genomic library enriched with tandem repeat motifs, we identified 16 primer pairs, and characterized them in two populations. Nine primers amplified the expected size fragments and seven SSRs were polymorphic, providing a total of 38 alleles and an average of 4.22 alleles per marker. The polymorphic information content (PIC) ranged from 0.44 to 0.94 with an average of 0.65. The average observed heterozygosity across all loci varied from 0.61 to 1.00. The observed (H O ) and expected (H E ) heterozygosity within the two populations varied from 0.65 to 1.00 and from 0.31 to 1.00, respectively. Conclusions: These newly developed SSR markers are a powerful tool for population genetic analyses and may be useful in studies on species ecology, evolution, and taxonomy.-
Descrição: dc.descriptionDepartment of Forest Science São Paulo State University UNESP-
Descrição: dc.descriptionDepartment of Forest Sciences Laboratory of Tree Genetic Conservation Federal University of Lavras UFLA-
Descrição: dc.descriptionDepartment of Biology Federal University of Minas Gerais UFMG-
Descrição: dc.descriptionDepartment of Forest Science São Paulo State University UNESP-
Idioma: dc.languageen-
Relação: dc.relationBiological Research-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectEremanthus erythropappus-
Palavras-chave: dc.subjectEremanthus glomerulatus-
Palavras-chave: dc.subjectEremanthus incanus-
Palavras-chave: dc.subjectGenetic diversity-
Palavras-chave: dc.subjectSimple sequence repeats-
Título: dc.titleDevelopment and characterization of nuclear microsatellite markers for Eremanthus erythropappus and their transferability across related species-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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