Cannabis-like activity of Zornia latifolia Sm. detected in vitro on rat cortical neurons: major role of the flavone syzalterin

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Autor(es): dc.contributorETT Spa-
Autor(es): dc.contributorInstitute of Biophysics-
Autor(es): dc.contributorUniversity of Eastern Piedmont-
Autor(es): dc.contributorUniversity of Genova-
Autor(es): dc.contributorItalian National Police-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorAlloisio, Susanna-
Autor(es): dc.creatorClericuzio, Marco-
Autor(es): dc.creatorNobile, Mario-
Autor(es): dc.creatorSalis, Annalisa-
Autor(es): dc.creatorDamonte, Gianluca-
Autor(es): dc.creatorCanali, Claudia-
Autor(es): dc.creatorFortuna-Perez, Ana Paula [UNESP]-
Autor(es): dc.creatorCornara, Laura-
Autor(es): dc.creatorBurlando, Bruno-
Data de aceite: dc.date.accessioned2022-02-22T00:26:25Z-
Data de disponibilização: dc.date.available2022-02-22T00:26:25Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2019-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1080/01480545.2020.1788057-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/199084-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/199084-
Descrição: dc.descriptionZornia latifolia is a plant suspected to possess psychoactive properties and marketed as a marijuana substitute under the name ‘maconha brava’. In this study, the effects of fractions obtained from a 2-propanol extract of aerial portions of the plant were determined by multielectrode array (MEA) analyses on cultured networks of rat cortical neurons. Lipophilic (ZL_lipo, mainly containing flavonoid aglycones), and hydrophilic (ZL_hydro, mainly containing flavonoid glycosides) fractions were initially obtained from the raw extract. ZL_lipo significantly inhibited mean firing rate (MFR) and mean bursting rate (MBR) of MEA recordings, while ZL_hydro induced no inhibition. Column chromatography separation of ZL_lipo yielded five fractions (ZL1-ZL5), among which ZL1 induced the strongest MFR and MBR inhibition. NMR and HPLC-MS analyses of ZL1 revealed the prevalence of the common flavonoids genistein (1) and apigenin (2) (in about a 1:1 ratio), and the presence of the rare flavone syzalterin (6,8-dimethylapigenin) (3) as a minor compound. Exposures of MEA to apigenin and genistein standards did not induce the MFR and MBR inhibition observed with ZL1, whereas exposure to syzalterin standard or to a 1:9 mixture syzalterin-genistein induced effects similar to ZL1. These inhibitory effects were comparable to that observed with high-THC hashish, possibly accounting for the plant psychoactive properties. Data indicate that Z. latifolia, currently marketed as a free herbal product, should be subjected to measures of control. In addition, syzalterin showed distinctive pharmacological properties, opening the way to its possible exploitation as a neuroactive drug.-
Descrição: dc.descriptionETT Spa-
Descrição: dc.descriptionNational Research Council (CNR) Institute of Biophysics-
Descrição: dc.descriptionDepartment of Sciences and Technological Innovation (DISIT) University of Eastern Piedmont-
Descrição: dc.descriptionCenter of Excellence for Biomedical Research (CEBR) University of Genova-
Descrição: dc.descriptionDepartment of Experimental Medicine (DIMES) Section of Biochemistry University of Genova-
Descrição: dc.descriptionForensic Science Police Service Italian National Police-
Descrição: dc.descriptionDepartment of Botany São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Earth Environment and Life Sciences (DISTAV) University of Genova-
Descrição: dc.descriptionDepartment of Pharmacy (DIFAR) University of Genova-
Descrição: dc.descriptionDepartment of Botany São Paulo State University (UNESP)-
Idioma: dc.languageen-
Relação: dc.relationDrug and Chemical Toxicology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectFlavonoids-
Palavras-chave: dc.subjecthashish-
Palavras-chave: dc.subjectmultielectrode array-
Palavras-chave: dc.subjectpsychoactive metabolites-
Palavras-chave: dc.subjectsmart drugs-
Título: dc.titleCannabis-like activity of Zornia latifolia Sm. detected in vitro on rat cortical neurons: major role of the flavone syzalterin-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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