Fungistatic action of N-acetylcysteine on candida albicans biofilms and its interaction with antifungal agents

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorNunes, Thaís Soares Bezerra Santos [UNESP]-
Autor(es): dc.creatorRosa, Leticia Matheus [UNESP]-
Autor(es): dc.creatorVega-Chacón, Yuliana [UNESP]-
Autor(es): dc.creatorMima, Ewerton Garcia de Oliveira [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:26:21Z-
Data de disponibilização: dc.date.available2022-02-22T00:26:21Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-07-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/microorganisms8070980-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/199060-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/199060-
Descrição: dc.descriptionTherapies targeted to fungal biofilms, mainly against the matrix, and therapies that do not induce microbial resistance are relevant. N-acetylcysteine (NAC), a mucolytic agent, has shown antimicrobial action. This study evaluated the effect of NAC against fluconazole-susceptible (CaS) and-resistant (CaR) Candida albicans. The susceptibility of planktonic cultures to NAC, the effect of NAC on biofilms and their matrix, the interaction of NAC with antifungal agents, and confocal microscopy were evaluated. Data were analyzed descriptively and by the ANOVA/Welch and Tukey/Gomes–Howell tests. The minimum inhibitory concentration (MIC) of NAC was 25 mg/mL for both strains. NAC significantly reduced the viability of both fungal strains. Concentrations higher than the MIC (100 and 50 mg/mL) reduced the viability and the biomass. NAC at 12.5 mg/mL increased the fungal viability. NAC also reduced the soluble components of the biofilm matrix, and showed synergism with caspofungin against planktonic cultures of CaS, but not against biofilms. Confocal images demonstrated that NAC reduced the biofilm thickness and the fluorescence intensity of most fluorochromes used. High concentrations of NAC had similar fungistatic effects against both strains, while a low concentration showed the opposite result. The antibiofilm action of NAC was due to its fungistatic action.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionLaboratory of Applied Microbiology Department of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)-
Descrição: dc.descriptionLaboratory of Applied Microbiology Department of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)-
Descrição: dc.descriptionFAPESP: 2018/02513-9-
Formato: dc.format1-27-
Idioma: dc.languageen-
Relação: dc.relationMicroorganisms-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAcetylcysteine-
Palavras-chave: dc.subjectAntifungal agents-
Palavras-chave: dc.subjectBiofilms-
Palavras-chave: dc.subjectCandida albicans-
Palavras-chave: dc.subjectDrug resistance-
Palavras-chave: dc.subjectExtracellular matrix-
Título: dc.titleFungistatic action of N-acetylcysteine on candida albicans biofilms and its interaction with antifungal agents-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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