Point-of-care testing for measuring haemolymph glucose in invertebrates is not a validmethod

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorPrincipe, Silas C. [UNESP]-
Autor(es): dc.creatorAugusto, Alessandra [UNESP]-
Autor(es): dc.creatorCosta, Tânia M. [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:26:00Z-
Data de disponibilização: dc.date.available2022-02-22T00:26:00Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2019-01-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1093/conphys/coz079-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/198952-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/198952-
Descrição: dc.descriptionBlood glucose is widely used as a physiological parameter for vertebrates and invertebrates. However, its measurement in the field is often difficult due to the need for expensive and non-portable equipment. Point-of-care (POC) devices, originally intended for human use, are increasingly being used for measuring blood parameters of animals in the field. In this regard, POC glucose meters are becoming valuable tools for conservation physiologists, as glucose can be a useful indicator of stress response. In invertebrates, the use of POC glucose meters is still scarce, and no study yet has evaluated their usability in crustaceans and molluscs.We tested if a POC device can be used to measure haemolymph glucose in two widely used models, Leptuca thayeri and Perna perna, compared with a standard laboratory method. The device was unable to measure glucose in P. perna haemolymph due to equipment inaccuracy and low glucose concentration in this species (10.13±6.25 mg/dL). Additionally, despite the device being capable of measuring glucose in L. thayeri haemolymph, Bland-Altman plots showed a strong bias and wide limits of agreement, and Lin's concordance correlation coefficient showed a weak concordance between methods. When simulating experimental conditions, POC results differed from those found using the standard method. We conclude that POC glucose meters are unsuitable for assessing glucose in mussels and should not be used in crabs as results are inaccurate.-
Descrição: dc.descriptionSão Paulo State University (UNESP) Biosciences Institute Botucatu Campus, R. Prof. Dr. Antônio Celso 250-
Descrição: dc.descriptionSão Paulo State University (UNESP) Biosciences Institute Coastal Campus, Praça Infante Dom Henrique s/n-
Descrição: dc.descriptionSão Paulo State University (UNESP) CAUNESP Prof. Paulo Donato Castellane S/n-
Descrição: dc.descriptionSão Paulo State University (UNESP) Biosciences Institute Botucatu Campus, R. Prof. Dr. Antônio Celso 250-
Descrição: dc.descriptionSão Paulo State University (UNESP) Biosciences Institute Coastal Campus, Praça Infante Dom Henrique s/n-
Descrição: dc.descriptionSão Paulo State University (UNESP) CAUNESP Prof. Paulo Donato Castellane S/n-
Idioma: dc.languageen-
Relação: dc.relationConservation Physiology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectFiddler crab-
Palavras-chave: dc.subjectGlucose-
Palavras-chave: dc.subjectLeptuca thayeri-
Palavras-chave: dc.subjectMolluscs-
Palavras-chave: dc.subjectPerna perna-
Palavras-chave: dc.subjectPortable meter-
Título: dc.titlePoint-of-care testing for measuring haemolymph glucose in invertebrates is not a validmethod-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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