Anthraquinone encapsulation into polymeric nanocapsules as a new drug from biotechnological origin designed for photodynamic therapy

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.contributorFederal University of Sao Francisco Valley - UNIVASF-
Autor(es): dc.creatorAmantino, Camila F. [UNESP]-
Autor(es): dc.creatorde Baptista-Neto, Álvaro [UNESP]-
Autor(es): dc.creatorBadino, Alberto C.-
Autor(es): dc.creatorSiqueira-Moura, Marigilson P.-
Autor(es): dc.creatorTedesco, Antonio C.-
Autor(es): dc.creatorPrimo, Fernando L. [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:25:55Z-
Data de disponibilização: dc.date.available2022-02-22T00:25:55Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-09-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.pdpdt.2020.101815-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/198927-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/198927-
Descrição: dc.descriptionPhotodynamic therapy has been applied for the treatment of many diseases, especially skin diseases. However, poor aqueous solubility and toxicity of some photosensitizer drugs are the main disadvantages for their direct clinical applications. Thus, biotechnology and nanotechnology are important tools in the development of new ways of obtaining photoactive compounds that are biocompatible. We investigated the potential of a new nanostructured photosensitizer, an anthraquinone derivative produced by biotechnological process; then we associated nanotechnology to obtain a nanostructured anthraquinone active molecule. For this, it was prepared a classical nanocapsule formulations containing poly(lactide-co-glycolide) (PLGA) coating for encapsulation of anthraquinone derivative. These formulations were characterized by their physicochemical, morphological, photophysical properties, and stability. We performed in vitro biocompatibility and photodynamic activity assays of free and nanostructured anthraquinone. Nanocapsule formulations containing anthraquinone derivative showed a nanometric profile with particle size around 250 nm, negative zeta potential around −30 mV, and partially monodisperse. Besides that, characteristic spherical morphology of nanocapsules and homogeneous particle surface were observed by AFM analyses. The in vitro biocompatibility assay showed absence of cytotoxicity for all tested RD/NC concentrations and also for unloaded/NC in NIH3T3 cells. In vitro photoactivation assay using NIH3T3 cells showed that nanocapsules promoted greater drug uptake by NIH3T3 cells, around of 87%, of cell death compared to free drug showed around 48% of cell death. The anthraquinone derivative showed potential for use in PDT. Besides the association with nanocapsules improved cell uptake of photosensitizer resulting in increased cell death compared to free anthraquinone.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionDepartment of Engineering of Bioprocess and Biotechnology School of Pharmaceutical Sciences São Paulo State University - UNESP-
Descrição: dc.descriptionDepartment of Chemistry Center of Nanotechnology and Tissue Engineering – Photobiology and Photomedicine Research Group Faculty of Philosophy Sciences and Letters of Ribeirão Preto University of São Paulo - USP-
Descrição: dc.descriptionGraduate Program of Chemical Engineering Federal University of São Carlos-
Descrição: dc.descriptionCollege of Pharmaceutical Sciences Federal University of Sao Francisco Valley - UNIVASF-
Descrição: dc.descriptionDepartment of Engineering of Bioprocess and Biotechnology School of Pharmaceutical Sciences São Paulo State University - UNESP-
Idioma: dc.languageen-
Relação: dc.relationPhotodiagnosis and Photodynamic Therapy-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAnthraquinone-
Palavras-chave: dc.subjectBiotechnological photosensitizers-
Palavras-chave: dc.subjectPhotodynamic therapy-
Palavras-chave: dc.subjectPLGA-nanocapsule-
Palavras-chave: dc.subjectPolymeric nanomaterials-
Título: dc.titleAnthraquinone encapsulation into polymeric nanocapsules as a new drug from biotechnological origin designed for photodynamic therapy-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.