A modular autoinduction device for control of gene expression in Bacillus subtilis

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorInstitute for Technical Microbiology-
Autor(es): dc.creatorCorrêa, Graciely Gomes [UNESP]-
Autor(es): dc.creatorLins, Milca Rachel da Costa Ribeiro [UNESP]-
Autor(es): dc.creatorSilva, Bruna Fernandes [UNESP]-
Autor(es): dc.creatorde Paiva, Gabriela Barbosa [UNESP]-
Autor(es): dc.creatorZocca, Vitoria Fernanda Bertolazzi [UNESP]-
Autor(es): dc.creatorRibeiro, Nathan Vinicius [UNESP]-
Autor(es): dc.creatorPicheli, Flavio Pereira [UNESP]-
Autor(es): dc.creatorMack, Matthias-
Autor(es): dc.creatorPedrolli, Danielle Biscaro [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:25:51Z-
Data de disponibilização: dc.date.available2022-02-22T00:25:51Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-09-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.ymben.2020.03.012-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/198900-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/198900-
Descrição: dc.descriptionIntense synthesis of proteins and chemicals in engineered microbes impose metabolic burden, frequently leading to reduced growth and heterogeneous cell population. Thus, the correct balance between growth and production is important. Such balance can be engineered through dynamic control of pathways, but few broadly applicable tools are available to achieve this. We present an autonomous control of gene expression mediated by quorum sensing in Bacillus subtilis, able to self-monitor and induce expression without human supervision. Two variations of the induction module and seven of the response module were engineered generating a range of induction folds and strengths for gene expression control. Our strongest response promoter is 2.5 and 3.2 times stronger than the well-characterized promoters PsrfA and Pveg, respectively. We applied our strongest autoinduction device for the production of the vitamin B2. This study presents a toolbox of autoinduction modules for B. subtilis that is modular and tunable.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionUniversidade Estadual Paulista (UNESP) School of Pharmaceutical Sciences Department of Bioprocess Engineering and Biotechnology, Rodovia Araraquara-Jau Km1-
Descrição: dc.descriptionMannheim University of Applied Sciences Institute for Technical Microbiology, Paul-Wittsack-Str. 10-
Descrição: dc.descriptionUniversidade Estadual Paulista (UNESP) School of Pharmaceutical Sciences Department of Bioprocess Engineering and Biotechnology, Rodovia Araraquara-Jau Km1-
Descrição: dc.descriptionCAPES: 001-
Descrição: dc.descriptionFAPESP: 2014/17564–7-
Descrição: dc.descriptionCAPES: CAPES SWE 290109/2017–5-
Formato: dc.format326-334-
Idioma: dc.languageen-
Relação: dc.relationMetabolic Engineering-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAutoinduction-
Palavras-chave: dc.subjectBacillus subtilis-
Palavras-chave: dc.subjectDynamic regulation-
Palavras-chave: dc.subjectLuxR/I-
Palavras-chave: dc.subjectQuorum sensing-
Palavras-chave: dc.subjectSynthetic biology-
Título: dc.titleA modular autoinduction device for control of gene expression in Bacillus subtilis-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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