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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.contributor | University of Magdalena | - |
Autor(es): dc.creator | Pedraza, Midian C. Castillo [UNESP] | - |
Autor(es): dc.creator | De Oliveira Fratucelli, Erick Dante [UNESP] | - |
Autor(es): dc.creator | Ribeiro, Sabrina Marcela [UNESP] | - |
Autor(es): dc.creator | Salamanca, Elkin Jahir Florez [UNESP] | - |
Autor(es): dc.creator | Da Silva Colin, Jaqueline [UNESP] | - |
Autor(es): dc.creator | Klein, Marlise I. [UNESP] | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:25:37Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:25:37Z | - |
Data de envio: dc.date.issued | 2020-12-11 | - |
Data de envio: dc.date.issued | 2020-12-11 | - |
Data de envio: dc.date.issued | 2020-05-01 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.3390/molecules25092232 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/198825 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/198825 | - |
Descrição: dc.description | Dental caries is a diet-biofilm-dependent disease. Streptococcus mutans contributes to cariogenic biofilms by producing an extracellular matrix rich in exopolysaccharides and acids. The study aimed to determine the effect of topical treatments with compound 1771 (modulates lipoteichoic acid (LTA) metabolism) and myricetin (affects the synthesis of exopolysaccharides) on S. mutans biofilms. In vitro S. mutans UA159 biofilms were grown on saliva-coated hydroxyapatite discs, alternating 0.1% sucrose and 0.5% sucrose plus 1% starch. Twice-daily topical treatments were performed with both agents alone and combined with and without fluoride: compound 1771 (2.6 μg/mL), myricetin (500 μg/mL), 1771 + myricetin, fluoride (250 ppm), 1771 + fluoride, myricetin + fluoride, 1771 + myricetin + fluoride, and vehicle. Biofilms were evaluated via microbiological, biochemical, imaging, and gene expression methods. Compound 1771 alone yielded less viable counts, biomass, exopolysaccharides, and extracellular LTA. Moreover, the combination 1771 + myricetin + fluoride decreased three logs of bacterium counts, 60% biomass, >74% exopolysaccharides, and 20% LTA. The effect of treatments on extracellular DNA was not pronounced. The combination strategy affected the size of microcolonies and exopolysaccharides distribution and inhibited the expression of genes linked to insoluble exopolysaccharides synthesis. Therefore, compound 1771 prevented the accumulation of S. mutans biofilm; however, the effect was more pronounced when it was associated with fluoride and myricetin. | - |
Descrição: dc.description | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | - |
Descrição: dc.description | Department of Dental Materials and Prosthodontics School of Dentistry Sao Paulo State University (UNESP), Rua Humaita, 1680 | - |
Descrição: dc.description | Faculty of Health Sciences University of Magdalena | - |
Descrição: dc.description | Department of Dental Materials and Prosthodontics School of Dentistry Sao Paulo State University (UNESP), Rua Humaita, 1680 | - |
Descrição: dc.description | FAPESP: #2014/05423-0 | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Molecules | - |
???dc.source???: dc.source | Scopus | - |
Palavras-chave: dc.subject | Biofilm | - |
Palavras-chave: dc.subject | Compound 1771 | - |
Palavras-chave: dc.subject | Exopolysaccharides | - |
Palavras-chave: dc.subject | Lipoteichoic acids | - |
Palavras-chave: dc.subject | Myricetin | - |
Palavras-chave: dc.subject | Streptococcusmutans | - |
Título: dc.title | Modulation of lipoteichoic acids and exopolysaccharides prevents Streptococcus mutans biofilm accumulation | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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