Efficient isolation and proliferation of human adipose-derived mesenchymal stromal cells in xeno-free conditions

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorInstitute of Medical-Hospital Service (IAM)-
Autor(es): dc.contributorSão Lucas – Cell Therapy Group-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorFuoco, Natalia Langenfeld-
Autor(es): dc.creatorde Oliveira, Rafael Guilen-
Autor(es): dc.creatorMarcelino, Monica Yonashiro-
Autor(es): dc.creatorStessuk, Talita-
Autor(es): dc.creatorSakalem, Marna Eliana [UNESP]-
Autor(es): dc.creatorMedina, Denis Aloisio Lopes-
Autor(es): dc.creatorModotti, Waldir Pereira-
Autor(es): dc.creatorForte, Andresa-
Autor(es): dc.creatorRibeiro-Paes, João Tadeu [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:25:04Z-
Data de disponibilização: dc.date.available2022-02-22T00:25:04Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-04-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s11033-020-05322-9-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/198629-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/198629-
Descrição: dc.descriptionClassical methods used for culture of adipose-derived mesenchymal stromal cells (ADSCs) use xenobiotic components, which may present a potential risk for biological contamination and/or elicit immunological reactions. Therefore, the aim of this study was to establish a xeno-free methodology for the isolation and proliferation of human ADSCs (hADSCs). hADSCs were isolated by enzymatic digestion or mechanical dissociation and cultured in the presence of fetal bovine serum or human platelet lysate. Proliferation curves were performed as a function of time from the cell culture and used to calculate the population doubling time. Immunophenotyping and differentiation tests were used to identify and characterize the hADSCs. Human ADSCs isolated and cultured in conventional or xenobiotic-free conditions peaked at different days but achieved similar maximum proliferation. The hADSCs differentiation ability was similar in all groups. The characterization of hADSCs by flow cytometry showed low contamination of the cultures by other cell types. The xenobiotic-free methodology described in this study is a feasible and reproducible alternative for isolation and proliferation of hADSCs. This methodology is in accordance with the recommendations of the National Health Surveillance Agency, which proposes avoidance of xenobiotic products.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionBiotechnology Interunits Post-Graduation Program Biomedical Science Institute University of São Paulo (USP)-
Descrição: dc.descriptionInstitute of Medical-Hospital Service (IAM)-
Descrição: dc.descriptionSão Lucas – Cell Therapy Group-
Descrição: dc.descriptionGenetics and Cell Therapy Laboratory (GenTe Cel) São Paulo State University (Unesp)-
Descrição: dc.descriptionLaboratório de Genética e Terapia Celular – GenTe Cel Departamento de Biotecnologia – Unesp, Av. Dom Antonio, 2100-
Descrição: dc.descriptionGenetics and Cell Therapy Laboratory (GenTe Cel) São Paulo State University (Unesp)-
Descrição: dc.descriptionLaboratório de Genética e Terapia Celular – GenTe Cel Departamento de Biotecnologia – Unesp, Av. Dom Antonio, 2100-
Formato: dc.format2475-2486-
Idioma: dc.languageen-
Relação: dc.relationMolecular Biology Reports-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAdipose tissue-
Palavras-chave: dc.subjectFetal bovine serum (FBS)-
Palavras-chave: dc.subjectMesenchymal stromal cells-
Palavras-chave: dc.subjectPlatelet lysate-
Palavras-chave: dc.subjectStem cells-
Título: dc.titleEfficient isolation and proliferation of human adipose-derived mesenchymal stromal cells in xeno-free conditions-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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