A novel multiple reactor system for the long-term production of L-asparaginase by Penicillium sp. LAMAI 505

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Autor(es): dc.contributorUniversidade Federal de Uberlândia (UFU)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorVieira, William Fernando-
Autor(es): dc.creatorCorrea, Higor Túlio-
Autor(es): dc.creatorSilveira Campos, Edgar-
Autor(es): dc.creatorSette, Lara D [UNESP]-
Autor(es): dc.creatorPessoa, Adalberto-
Autor(es): dc.creatorCardoso, Vicelma Luiz-
Autor(es): dc.creatorCoutinho Filho, Ubirajara-
Data de aceite: dc.date.accessioned2022-02-22T00:24:59Z-
Data de disponibilização: dc.date.available2022-02-22T00:24:59Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-03-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.procbio.2019.11.012-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/198593-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/198593-
Descrição: dc.descriptionIn this paper a novel system of multiple reactors with immobilized cells (MRICs) to produce L-asparaginase by Penicillium sp. LAMAI 505 is presented. The effects of the number of associated reactors, nutrient composition, residence time for nutrient cycling, and extraction cycles were investigated using an artificial neural network (ANN). The ANN model was satisfactory (R² > 0.85 with an error lower than 2.4%) and the MRICs performed well when compared to the literature. The cyclic operation of the MRICs can regenerate cells within the inert support, allowing for the long-term production of L-asparaginase. L-asparaginase activity of 13.7 U/gds was achieved under optimised conditions that included a residence time of 33.5 min, pH of 5.1, and concentrations of L-asparagine and glucose of 1.2 and 3.0 g/L, respectively. Furthermore, we demonstrate that MRICs are effective with other fungi and can be used for other products.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)-
Descrição: dc.descriptionFaculty of Chemical Engineering Federal University of Uberlândia Campus Santa Monica, Av. João Naves de Ávila, 2121, Bloco 1K, 38.400-902-
Descrição: dc.descriptionDepartment of Biochemical and Pharmaceutical Technology Faculty of Pharmaceutical Sciences University of São Paulo (USP)-
Descrição: dc.descriptionInstitute of Biotechnology Federal University of Uberlândia-
Descrição: dc.descriptionDepartment of Biochemical and Microbiology University Estadual Paulista Júlio Mesquita (UNESP)-
Descrição: dc.descriptionDepartment of Biochemical and Microbiology University Estadual Paulista Júlio Mesquita (UNESP)-
Descrição: dc.descriptionFAPEMIG: APQ – 03927-16-
Formato: dc.format23-31-
Idioma: dc.languageen-
Relação: dc.relationProcess Biochemistry-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectL-asparaginase-
Palavras-chave: dc.subjectNovel bioreactor-
Palavras-chave: dc.subjectSolid-State fermentation-
Título: dc.titleA novel multiple reactor system for the long-term production of L-asparaginase by Penicillium sp. LAMAI 505-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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