High genomic variability in equine infectious anemia virus obtained from naturally infected horses in Pantanal, Brazil: An endemic region case

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Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade Federal de Viçosa (UFV)-
Autor(es): dc.contributorEvandro Chagas Institute-
Autor(es): dc.contributorUniversidade Federal de Minas Gerais (UFMG)-
Autor(es): dc.contributorMato Grosso Federal University-
Autor(es): dc.contributorEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)-
Autor(es): dc.creatorMalossi, Camila Dantas [UNESP]-
Autor(es): dc.creatorFioratti, Eduardo Gorzoni-
Autor(es): dc.creatorCardoso, Jedson Ferreira-
Autor(es): dc.creatorMagro, Angelo Jose [UNESP]-
Autor(es): dc.creatorKroon, Erna Geessien-
Autor(es): dc.creatorde Aguiar, Daniel Moura-
Autor(es): dc.creatorCosta Marque Borges, Alice Mamede-
Autor(es): dc.creatorNogueira, Marcia Furlan-
Autor(es): dc.creatorUllmann, Leila Sabrina [UNESP]-
Autor(es): dc.creatorAraujo, João Pessoa [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:24:47Z-
Data de disponibilização: dc.date.available2022-02-22T00:24:47Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-02-11-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/v12020207-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/198531-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/198531-
Descrição: dc.descriptionEquine infectious anemia virus (EIAV) is a persistent lentivirus that causes equine infectious anemia (EIA). In Brazil, EIAV is endemic in the Pantanal region, and euthanasia is not mandatory in this area. All of the complete genomic sequences from field viruses are from North America, Asia, and Europe, and only proviral genomic sequences are available. Sequences from Brazilian EIAV are currently available only for gag and LTR regions. Thus, the present study aimed for the first time to sequence the entire EIAV genomic RNA in naturally infected horses from an endemic area in Brazil. RNA in plasma from naturally infected horses was used for next-generation sequencing (NGS), and gaps were filled using Sanger sequencing methodology. Complete viral genomes of EIAV from two horses were obtained and annotated (Access Number: MN560970 and MN560971). Putative genes were analyzed and compared with previously described genes, showing conservation in gag and pol genes and high variations in LTR and env sequences. Amino acid changes were identified in the p26 protein, one of the most common targets used for diagnosis, and p26 molecular modelling showed surface amino acid alterations in some epitopes. Brazilian genome sequences presented 88.6% nucleotide identity with one another and 75.8 to 77.3% with main field strains, such as EIAV Liaoning, Wyoming, Ireland, and Italy isolates. Furthermore, phylogenetic analysis suggested that this Brazilian strain comprises a separate monophyletic group. These results may help to better characterize EIAV and to overcome the challenges of diagnosing and controlling EIA in endemic regions.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionInstitute for Biotechnology São Paulo State University (Unesp)-
Descrição: dc.descriptionAgrarian Sciences Institute (ICA) Vales do Jequitinhonha e Mucuri Federal University (UFVJM)-
Descrição: dc.descriptionCenter for Technological Innovation Evandro Chagas Institute-
Descrição: dc.descriptionVirology Laboratory Universidade Federal de Minas Gerais-
Descrição: dc.descriptionVirology and Rickettsiosis Laboratory Mato Grosso Federal University-
Descrição: dc.descriptionEMBRAPA Pantanal-
Descrição: dc.descriptionInstitute for Biotechnology São Paulo State University (Unesp)-
Descrição: dc.descriptionFAPESP: 2014/13532-3-
Idioma: dc.languageen-
Relação: dc.relationViruses-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectEndemic region-
Palavras-chave: dc.subjectEquine-
Palavras-chave: dc.subjectEquine infectious anemia virus-
Palavras-chave: dc.subjectMolecular characterization-
Palavras-chave: dc.subjectRNA genome-
Título: dc.titleHigh genomic variability in equine infectious anemia virus obtained from naturally infected horses in Pantanal, Brazil: An endemic region case-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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