Rainbow trout slow myoblast cell culture as a model to study slow skeletal muscle, and the characterization of mir-133 and mir-499 families as a case study

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorSchool of Biology-
Autor(es): dc.contributorPhysiology and Immunology-
Autor(es): dc.creatorda Silva Duran, Bruno Oliveira [UNESP]-
Autor(es): dc.creatorDal-Pai-Silva, Maeli [UNESP]-
Autor(es): dc.creatorde la Serrana, Daniel Garcia-
Data de aceite: dc.date.accessioned2022-02-22T00:24:37Z-
Data de disponibilização: dc.date.available2022-02-22T00:24:37Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2019-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1242/jeb.216390-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/198465-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/198465-
Descrição: dc.descriptionMuscle fibres are classified as fast, intermediate and slow. In vitro myoblast cell culture model from fast muscle is a very useful tool to study muscle growth and development; however, similar models for slow muscle do not exist. Owing to the compartmentalization of fish muscle fibres, we have developed a slow myoblast cell culture for rainbow trout (Oncorhynchus mykiss). Slow and fast muscle-derived myoblasts have similar morphology, but with differential expression of slow muscle markers such as slow myhc, sox6 and pgc-1α. We also characterized the mir-133 and mir-499 microRNA families in trout slow and fast myoblasts as a case study during myogenesis and in response to electrostimulation. Three mir-133 (a-1a, a-1b and a-2) and four mir-499 (aa, ab, ba and bb) paralogues were identified for rainbow trout and named base on their phylogenetic relationship to zebrafish and Atlantic salmon orthologues. Omy-mir-499ab and omymir-499bb had 0.6 and 0.5-fold higher expression in slow myoblasts compared with fast myoblasts, whereas mir-133 duplicates had similar levels in both phenotypes and little variation during development. Slow myoblasts also showed increased expression for omy-mir-499b paralogues in response to chronic electrostimulation (7-fold increase for omy-mir-499ba and 2.5-fold increase for omy-mir-499bb). The higher expression of mir-499 paralogues in slow myoblasts suggests a role in phenotype determination, while the lack of significant differences of mir-133 copies during culture development might indicate a different role in fish compared with mammals. We have also found signs of sub-functionalization of mir-499 paralogues after electrostimulation, with omy-mir-499b copies more responsive to electrical signals.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionScottish Funding Council-
Descrição: dc.descriptionSaõ Paulo State University (UNESP) Institute of Biosciences Department of Morphology-
Descrição: dc.descriptionUniversity of St.Andrews Scottish Oceans Institute School of Biology-
Descrição: dc.descriptionUniversity of Barcelona Faculty of Biology Department of Cell Biology Physiology and Immunology-
Descrição: dc.descriptionSaõ Paulo State University (UNESP) Institute of Biosciences Department of Morphology-
Descrição: dc.descriptionFAPESP: 2015/03234-8-
Descrição: dc.descriptionFAPESP: 2016/05009-4-
Descrição: dc.descriptionFAPESP: 2016/19683-9-
Descrição: dc.descriptionFAPESP: 2019/01592-5-
Descrição: dc.descriptionCNPq: 302656/2015-4-
Descrição: dc.descriptionCNPq: 447233/2014-
Descrição: dc.descriptionScottish Funding Council: HR09011-
Idioma: dc.languageen-
Relação: dc.relationJournal of Experimental Biology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCell culture-
Palavras-chave: dc.subjectElectrostimulation-
Palavras-chave: dc.subjectMiRNA-
Palavras-chave: dc.subjectMyoblasts-
Palavras-chave: dc.subjectSlow skeletal muscle-
Título: dc.titleRainbow trout slow myoblast cell culture as a model to study slow skeletal muscle, and the characterization of mir-133 and mir-499 families as a case study-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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