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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.contributor | School of Biology | - |
Autor(es): dc.contributor | Physiology and Immunology | - |
Autor(es): dc.creator | da Silva Duran, Bruno Oliveira [UNESP] | - |
Autor(es): dc.creator | Dal-Pai-Silva, Maeli [UNESP] | - |
Autor(es): dc.creator | de la Serrana, Daniel Garcia | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:24:37Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:24:37Z | - |
Data de envio: dc.date.issued | 2020-12-11 | - |
Data de envio: dc.date.issued | 2020-12-11 | - |
Data de envio: dc.date.issued | 2019-12-31 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1242/jeb.216390 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/198465 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/198465 | - |
Descrição: dc.description | Muscle fibres are classified as fast, intermediate and slow. In vitro myoblast cell culture model from fast muscle is a very useful tool to study muscle growth and development; however, similar models for slow muscle do not exist. Owing to the compartmentalization of fish muscle fibres, we have developed a slow myoblast cell culture for rainbow trout (Oncorhynchus mykiss). Slow and fast muscle-derived myoblasts have similar morphology, but with differential expression of slow muscle markers such as slow myhc, sox6 and pgc-1α. We also characterized the mir-133 and mir-499 microRNA families in trout slow and fast myoblasts as a case study during myogenesis and in response to electrostimulation. Three mir-133 (a-1a, a-1b and a-2) and four mir-499 (aa, ab, ba and bb) paralogues were identified for rainbow trout and named base on their phylogenetic relationship to zebrafish and Atlantic salmon orthologues. Omy-mir-499ab and omymir-499bb had 0.6 and 0.5-fold higher expression in slow myoblasts compared with fast myoblasts, whereas mir-133 duplicates had similar levels in both phenotypes and little variation during development. Slow myoblasts also showed increased expression for omy-mir-499b paralogues in response to chronic electrostimulation (7-fold increase for omy-mir-499ba and 2.5-fold increase for omy-mir-499bb). The higher expression of mir-499 paralogues in slow myoblasts suggests a role in phenotype determination, while the lack of significant differences of mir-133 copies during culture development might indicate a different role in fish compared with mammals. We have also found signs of sub-functionalization of mir-499 paralogues after electrostimulation, with omy-mir-499b copies more responsive to electrical signals. | - |
Descrição: dc.description | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | - |
Descrição: dc.description | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | - |
Descrição: dc.description | Scottish Funding Council | - |
Descrição: dc.description | Saõ Paulo State University (UNESP) Institute of Biosciences Department of Morphology | - |
Descrição: dc.description | University of St.Andrews Scottish Oceans Institute School of Biology | - |
Descrição: dc.description | University of Barcelona Faculty of Biology Department of Cell Biology Physiology and Immunology | - |
Descrição: dc.description | Saõ Paulo State University (UNESP) Institute of Biosciences Department of Morphology | - |
Descrição: dc.description | FAPESP: 2015/03234-8 | - |
Descrição: dc.description | FAPESP: 2016/05009-4 | - |
Descrição: dc.description | FAPESP: 2016/19683-9 | - |
Descrição: dc.description | FAPESP: 2019/01592-5 | - |
Descrição: dc.description | CNPq: 302656/2015-4 | - |
Descrição: dc.description | CNPq: 447233/2014 | - |
Descrição: dc.description | Scottish Funding Council: HR09011 | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Journal of Experimental Biology | - |
???dc.source???: dc.source | Scopus | - |
Palavras-chave: dc.subject | Cell culture | - |
Palavras-chave: dc.subject | Electrostimulation | - |
Palavras-chave: dc.subject | MiRNA | - |
Palavras-chave: dc.subject | Myoblasts | - |
Palavras-chave: dc.subject | Slow skeletal muscle | - |
Título: dc.title | Rainbow trout slow myoblast cell culture as a model to study slow skeletal muscle, and the characterization of mir-133 and mir-499 families as a case study | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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