Transcriptional patterns of Coffea arabica L. nitrate reductase, glutamine and asparagine synthetase genes are modulated under nitrogen suppression and coffee leaf rust

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Autor(es): dc.contributorUniversidade Estadual de Londrina (UEL)-
Autor(es): dc.contributorInstituto Agronômico Do Paraná-
Autor(es): dc.contributorInstituto Agronômico de Campinas-
Autor(es): dc.contributorUniversidade Do Oeste Paulista-
Autor(es): dc.contributorEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade Paranaense-
Autor(es): dc.creatorBaba, Viviane Yumi-
Autor(es): dc.creatorBraghini, Masako Toma-
Autor(es): dc.creatorDos Santos, Tiago Benedito-
Autor(es): dc.creatorDe Carvalho, Kenia-
Autor(es): dc.creatorSoares, João Danillo Moura-
Autor(es): dc.creatorIvamoto-Suzuki, Suzana Tiemi [UNESP]-
Autor(es): dc.creatorMaluf, Mirian P.-
Autor(es): dc.creatorPadilha, Lilian-
Autor(es): dc.creatorPaccola-Meirelles, Luzia D.-
Autor(es): dc.creatorPereira, Luiz Filipe-
Autor(es): dc.creatorDomingues, Douglas S. [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:24:33Z-
Data de disponibilização: dc.date.available2022-02-22T00:24:33Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2019-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.7717/peerj.8320-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/198434-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/198434-
Descrição: dc.descriptionThis study evaluated the transcriptional profile of genes related to nitrogen (N) assimilation in coffee plants susceptible and resistant to rust fungi under N sufficiency and N suppression. For this purpose, we inoculated young coffee leaves with Hemileia vastatrix uredospores and collected them at 0, 12, 24 and 48 hours post-inoculation (HPI) to evaluate the relative expressions of genes encoding cytosolic glutamine synthetase (CaGS1), plastid glutamine synthetase (CaGS2), nitrate reductase (CaNR), and asparagine synthetase (CaAS). The genes exhibited distinct patterns of transcriptional modulation for the different genotypes and N nutritional regimes. The resistant genotype (I59) presented high levels of transcription in response to pathogen inoculation for CaNR and CaGS1 genes, evaluated under N sufficiency in the initial moments of infection (12 HPI). The gene CaGS1 also showed a peak at 48 HPI. The susceptible genotype (CV99) showed increased transcript rates of CaNR at 12 and 24 HPI in response to rust inoculation. The transcriptional patterns observed for CV99, under N suppression, were high levels for CaAS and CaGS2 at all post-inoculation times in response to coffee leaf rust disease. In addition, CaGS1 was up-regulated at 48 HPI for CV99. Cultivar I59 showed high transcript levels at 12 HPI for CaAS and peaks at 24 and 48 HPI for CaGS2 in inoculated samples. Consequently, total chlorophyl concentration was influenced by N suppression and by rust infection. Regarding enzyme activities in vitro for glutamine synthetase and CaNR, there was an increase in infected coffee leaves (I59) and under N sufficiency. Moreover, CV99 was modulated in both N nutritional regimes for GS activity in response to rust. Our results indicate that N transport genes trigger a differential modulation between genotypes through the action of rust disease.-
Descrição: dc.descriptionDepartment of Agronomy Universidade Estadual de Londrina-
Descrição: dc.descriptionPlant Biotechnology Laboratory Instituto Agronômico Do Paraná-
Descrição: dc.descriptionCentro de Analise e Pesquisa Tecnologica Do Agronegocio Do Cafe 'Alcides Carvalho' Instituto Agronômico de Campinas-
Descrição: dc.descriptionPrograma de Pós-Graduação em Agronomia Universidade Do Oeste Paulista-
Descrição: dc.descriptionPlant Biotechnology Laboratory-
Descrição: dc.descriptionDepartment of Botany Instituto de Biociências São Paulo State University UNESP-
Descrição: dc.descriptionPlant Breeding-
Descrição: dc.descriptionDepartment of Agronomy Universidade Paranaense-
Descrição: dc.descriptionDepartment of Botany Instituto de Biociências São Paulo State University UNESP-
Idioma: dc.languageen-
Relação: dc.relationPeerJ-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectGene expression-
Palavras-chave: dc.subjectGenotypic response-
Palavras-chave: dc.subjectHemileia vastatrix-
Palavras-chave: dc.subjectMineral nutrition-
Palavras-chave: dc.subjectN assimilation-
Palavras-chave: dc.subjectRT-qPCR-
Título: dc.titleTranscriptional patterns of Coffea arabica L. nitrate reductase, glutamine and asparagine synthetase genes are modulated under nitrogen suppression and coffee leaf rust-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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