Rhipicephalus microplus cystatin as a potential cross-protective tick vaccine against Rhipicephalus appendiculatus

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorAvenida Bento Gonçalves-
Autor(es): dc.contributorInternational Livestock Research Institute (ILRI)-
Autor(es): dc.contributorUniversity of Nairobi-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorRua Sarmento Leite-
Autor(es): dc.contributorHokkaido University-
Autor(es): dc.contributorInstituto Nacional de Ciência e Tecnologia em Entomologia Molecular-
Autor(es): dc.creatorParizi, Luís Fernando-
Autor(es): dc.creatorRangel, Carolina Konrdörfer-
Autor(es): dc.creatorSabadin, Gabriela Alves-
Autor(es): dc.creatorSaggin, Bianca Fagundes-
Autor(es): dc.creatorKiio, Irene-
Autor(es): dc.creatorXavier, Marina Amaral-
Autor(es): dc.creatorda Silva Matos, Renata [UNESP]-
Autor(es): dc.creatorCamargo-Mathias, Maria Izabel [UNESP]-
Autor(es): dc.creatorSeixas, Adriana-
Autor(es): dc.creatorKonnai, Satoru-
Autor(es): dc.creatorOhashi, Kazuhiko-
Autor(es): dc.creatorGithaka, Naftaly Wang'ombe-
Autor(es): dc.creatorda Silva Vaz, Itabajara-
Data de aceite: dc.date.accessioned2022-02-22T00:24:29Z-
Data de disponibilização: dc.date.available2022-02-22T00:24:29Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-05-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.ttbdis.2020.101378-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/198423-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/198423-
Descrição: dc.descriptionRhipicephalus appendiculatus, the brown ear tick, is an important disease vector of livestock in eastern, central and southern Africa. Rhipicephalus appendiculatus acaricide resistance requires the search for alternative methods for its control. Cystatins constitute a superfamily of cysteine peptidase inhibitors vital for tick blood feeding and development. These inhibitors were proposed as antigens in anti-tick vaccines. In this work, we applied structural and biochemical approaches to characterize a new cystatin named R. appendiculatus cystatin 2a (Racys2a). Structural modeling showed that this new protein possesses characteristic type 2 cystatin motifs, besides conservation of other structural patterns along the protein. Peptidase inhibitory assays with recombinant Racys2a showed modulation of tick and host cathepsins involved in blood digestion and immune system responses, respectively. A heterologous tick challenge with R. appendiculatus in rabbits immunized with recombinant Rhipicephalus microplus cystatin 2c (rBmcys2c) was performed to determine cross-reactivity. Histological staining showed that rBmcys2c vaccination caused damage to the gut, salivary gland and ovary tissues in R. appendiculatus. Furthermore, cystatin vaccine reduced the number of fully engorged adult females in 11.5 %. Consequently, strategies to increase the protection rate are necessary, including the selection of two or more antigens to compose a vaccine cocktail.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)-
Descrição: dc.descriptionCentro de Biotecnologia Universidade Federal do Rio Grande do Sul Avenida Bento Gonçalves, 9500-
Descrição: dc.descriptionInternational Livestock Research Institute (ILRI), PO Box 30709-00100-
Descrição: dc.descriptionDepartment of Biochemistry School of Medicine University of Nairobi, P.O. Box 30197-00100-
Descrição: dc.descriptionDepartamento de Biologia Instituto de Biociências UNESP-Universidade Estadual Paulista-
Descrição: dc.descriptionDepartamento de Farmacociências Universidade Federal de Ciências da Saúde de Porto Alegre Rua Sarmento Leite, 245-
Descrição: dc.descriptionLaboratory of Infectious Diseases Department of Disease Control Graduate School of Veterinary Medicine Hokkaido University-
Descrição: dc.descriptionFaculdade de Veterinária Universidade Federal do Rio Grande do Sul Avenida Bento Gonçalves, 9090-
Descrição: dc.descriptionInstituto Nacional de Ciência e Tecnologia em Entomologia Molecular-
Descrição: dc.descriptionDepartamento de Biologia Instituto de Biociências UNESP-Universidade Estadual Paulista-
Idioma: dc.languageen-
Relação: dc.relationTicks and Tick-borne Diseases-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectParasite-
Palavras-chave: dc.subjectPeptidase inhibitor-
Palavras-chave: dc.subjectVaccination-
Título: dc.titleRhipicephalus microplus cystatin as a potential cross-protective tick vaccine against Rhipicephalus appendiculatus-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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