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Metadados | Descrição | Idioma |
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Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.creator | Machado, Felipe Paiva [UNESP] | - |
Autor(es): dc.creator | Khoury, Rayana Duarte [UNESP] | - |
Autor(es): dc.creator | Toia, Cassia Cestari [UNESP] | - |
Autor(es): dc.creator | Flores Orozco, Esteban Isai [UNESP] | - |
Autor(es): dc.creator | de Oliveira, Felipe Eduardo [UNESP] | - |
Autor(es): dc.creator | de Oliveira, Luciane Dias [UNESP] | - |
Autor(es): dc.creator | da Rosa Cardoso, Flávia Goulart [UNESP] | - |
Autor(es): dc.creator | Valera, Marcia Carneiro [UNESP] | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:24:25Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:24:25Z | - |
Data de envio: dc.date.issued | 2020-12-11 | - |
Data de envio: dc.date.issued | 2020-12-11 | - |
Data de envio: dc.date.issued | 2020-09-01 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1007/s00784-019-03191-6 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/198398 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/198398 | - |
Descrição: dc.description | Objectives: To compare the microbial load and composition and to determine the lipopolysaccharides (LPS) and lipoteichoic acid (LTA) concentrations found in primary apical periodontitis (PAP) and post-treatment apical periodontitis (PTAP), correlating these findings with clinical/tomographic features. Material and methods: Sixty patients with PAP (31) and PTAP (29) were submitted to clinical and tomographic assessment. Samples were collected from each root canal using paper points for microbiological assessment (culture technique and Checkerboard DNA-DNA hybridization) and determination of LPS and LTA levels (limulus amebocyte lysate and enzyme-linked immunosorbent assays, respectively). Data were correlated with clinical/tomographic findings and statistically analyzed using the Mann-Whitney and Pearson correlation tests (α = 5%). Results: A higher number of cultivable bacteria and LPS were found in PAP (p < 0.05). The median number of species per root canal found in PAP and PTAP was 9 and 22, respectively (p < 0.05). LPS was positively correlated with a larger periapical lesion volume (p <.05). LTA levels were similar in both infections and had no correlation with signs and symptoms. In PAP, gram-positive bacteria were correlated with spontaneous pain (p <.05) and exudate (p <.05). Tenderness to percussion and pain on palpation were correlated to the presence of both gram-positive and negative bacteria. In PTAP, a positive correlation was observed between both gram-positive and gram-negative bacteria with exudate and periapical lesion volume (p <.05). Conclusions: PAP had higher contents of microbial load and LPS compared with PTAP. However, PTAP presented a more diverse microbiota compared with PAP. Higher content of LPS was positively correlated with larger periapical bone destruction, whereas signs and symptoms with specific microorganisms. Clinical relevance: It was verified that PAP and PTAP are polymicrobial infections with predominance of gram-negative bacteria and a more diverse bacterial population found in PTAP. A wide interaction of specific microbial species resulted in different clinical features in both infections. | - |
Descrição: dc.description | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | - |
Descrição: dc.description | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | - |
Descrição: dc.description | Department of Restorative Dentistry Endodontic Division Institute of Science and Technology São Paulo State University (Unesp) | - |
Descrição: dc.description | Department of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (Unesp) | - |
Descrição: dc.description | Department of Restorative Dentistry Endodontic Division Institute of Science and Technology São Paulo State University (Unesp) | - |
Descrição: dc.description | Department of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (Unesp) | - |
Descrição: dc.description | CAPES: 001 | - |
Descrição: dc.description | FAPESP: 2015/05397-1 | - |
Descrição: dc.description | FAPESP: 2018/01703-9 | - |
Formato: dc.format | 3169-3179 | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Clinical Oral Investigations | - |
???dc.source???: dc.source | Scopus | - |
Palavras-chave: dc.subject | Apical periodontitis | - |
Palavras-chave: dc.subject | Endotoxins | - |
Palavras-chave: dc.subject | Enzyme-linked immunosorbent assay | - |
Palavras-chave: dc.subject | Lipoteichoic acid | - |
Palavras-chave: dc.subject | Microbiota | - |
Palavras-chave: dc.subject | Nucleic acid hybridization | - |
Título: dc.title | Primary versus post-treatment apical periodontitis: microbial composition, lipopolysaccharides and lipoteichoic acid levels, signs and symptoms | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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