Controlling the l-asparaginase extraction and purification by the appropriate selection of polymer/salt-based aqueous biphasic systems

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversity of Aveiro-
Autor(es): dc.creatorMagri, Agnes [UNESP]-
Autor(es): dc.creatorPimenta, Marcela V-
Autor(es): dc.creatorSantos, João HPM-
Autor(es): dc.creatorCoutinho, João AP-
Autor(es): dc.creatorVentura, Sónia PM-
Autor(es): dc.creatorMonteiro, Gisele-
Autor(es): dc.creatorRangel-Yagui, Carlota O-
Autor(es): dc.creatorPereira, Jorge FB [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:24:09Z-
Data de disponibilização: dc.date.available2022-02-22T00:24:09Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-04-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1002/jctb.6281-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/198302-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/198302-
Descrição: dc.descriptionBACKGROUND: l-Asparaginase (ASNase) is an important biopharmaceutical for the treatment of acute lymphoblastic leukemia (ALL); however, with some restrictions due to its high manufacturing costs. Aqueous biphasic systems (ABS) have been suggested as more economical platforms for the separation/purification of proteins, but a full understanding of the mechanisms behind the ASNase partition is still a major challenge. Polymer/salt-based ABS with different driving-forces (salting-out and hydrophilicity/hydrophobicity effects) were herein applied to control the partition of commercial ASNase. RESULTS: The main results showed the ASNase partition to the salt- or polymer-rich phase depending on the ABS studied, with extraction efficiencies higher than 95%. For systems composed of inorganic salts, the ASNase partition was controlled by the polyethylene glycol (PEG) molecular weight used. Cholinium-salts-based ABS were able to promote a preferential ASNase partition to the polymer-rich phase using PEG-600 and to the salt-rich phase using a more hydrophobic polypropylene glycol (PPG)-400 polymer. It was possible to select the ABS composed of PEG-2000 + potassium phosphate buffer as the most efficient to separate the ASNase from the main contaminant proteins (purification factor = 2.4 ± 0.2), while it was able to maintain the enzyme activity for posterior application as part of a therapeutic. CONCLUSION: Polymer/salt ABS can be used to control the partition of ASNase and adjust its purification yields, demonstrating the ABS potential as more economic platform for the selective recovery of therapeutic enzymes from complex broths. © 2019 Society of Chemical Industry.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionFundação para a Ciência e a Tecnologia-
Descrição: dc.descriptionDepartment of Bioprocesses and Biotechnology School of Pharmaceutical Sciences São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartamento de Tecnologia Bioquímico-Farmacêutica Faculdade de Ciências Farmacêuticas - Universidade de São Paulo São Paulo-
Descrição: dc.descriptionDepartment of Chemistry CICECO – Aveiro Institute of Materials University of Aveiro-
Descrição: dc.descriptionDepartment of Bioprocesses and Biotechnology School of Pharmaceutical Sciences São Paulo State University (UNESP)-
Descrição: dc.descriptionCAPES: 001-
Descrição: dc.descriptionCNPq: 163292/2015-9-
Descrição: dc.descriptionFAPESP: 2013/08617-7-
Descrição: dc.descriptionFAPESP: 2014/16424-7-
Descrição: dc.descriptionFAPESP: 2014/19793-3-
Descrição: dc.descriptionFAPESP: 2015/07749-2-
Descrição: dc.descriptionFAPESP: 2018/15104-0-
Descrição: dc.descriptionCNPq: 301832/201-0-
Descrição: dc.descriptionCNPq: 309595/2016-9-
Descrição: dc.descriptionFundação para a Ciência e a Tecnologia: IF/00402/2015-
Descrição: dc.descriptionFundação para a Ciência e a Tecnologia: SFRH/BD/102915/2014-
Descrição: dc.descriptionFundação para a Ciência e a Tecnologia: UID/CTM/50011/2019-
Formato: dc.format1016-1027-
Idioma: dc.languageen-
Relação: dc.relationJournal of Chemical Technology and Biotechnology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectaqueous biphasic systems (ABS)-
Palavras-chave: dc.subjectbioseparations-
Palavras-chave: dc.subjectenzymes-
Palavras-chave: dc.subjectliquid–liquid extraction-
Palavras-chave: dc.subjectpurification-
Palavras-chave: dc.subjectseparation-
Título: dc.titleControlling the l-asparaginase extraction and purification by the appropriate selection of polymer/salt-based aqueous biphasic systems-
Tipo de arquivo: dc.typelivro digital-
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