Quercetin pentaacetate inhibits in vitro human respiratory syncytial virus adhesion

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversity of Rome Tor Vergata-
Autor(es): dc.creatorLopes, Bruno Rafael Pereira [UNESP]-
Autor(es): dc.creatorda Costa, Mirian Feliciano [UNESP]-
Autor(es): dc.creatorde Genova Ribeiro, Amanda [UNESP]-
Autor(es): dc.creatorLima, Caroline Sprendel [UNESP]-
Autor(es): dc.creatorCaruso, Icaro Putinhon [UNESP]-
Autor(es): dc.creatorde Araújo, Gabriela Campos [UNESP]-
Autor(es): dc.creatorKubo, Letícia Hiromi [UNESP]-
Autor(es): dc.creatorIacovelli, Federico-
Autor(es): dc.creatorFalconi, Mattia-
Autor(es): dc.creatorDesideri, Alessandro-
Autor(es): dc.creatorde Oliveira, Juliana [UNESP]-
Autor(es): dc.creatorRegasini, Luis Octávio [UNESP]-
Autor(es): dc.creatorSouza, Fátima Pereira [UNESP]-
Autor(es): dc.creatorToledo, Karina Alves [UNESP]-
Autor(es): dc.creatorda Silva, Tiago Francisco [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:23:53Z-
Data de disponibilização: dc.date.available2022-02-22T00:23:53Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-01-14-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.virusres.2019.197805-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/198201-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/198201-
Descrição: dc.descriptionHuman respiratory syncytial virus (hRSV) is one of the main etiological agents of diseases of the lower respiratory tract and is often responsible for the hospitalization of children and the elderly. To date, treatments are only palliative and there is no vaccine available. Natural products show exceptional structural diversity and they have played a vital role in drug research. Several investigations focused on applied structural modification of natural products to improved metabolic stability, solubility and biological actions them. Quercetin is a flavonoid that presents several biological activities, including anti-hRSV role. Some works criticize the pharmacological use of Quercetin because it has low solubility and low specificity. In this sense, we acetylated Quercetin structure and we used in vitro and in silico assays to compare anti-hRSV function between Quercetin (Q0) and its derivative molecule (Q1). Q1 shows lower cytotoxic effect than Q0 on HEp-2 cells. In addition, Q1 was more efficient than Q0 to protect HEp-2 cells infected with different multiplicity of infection (0.1–1 MOI). The virucidal effects of Q0 and Q1 suggest interaction between these molecules and viral particle. Dynamic molecular results suggest that Q0 and Q1 may interact with F-protein on hRSV surface in an important region to adhesion and viral infection. Q1 interaction with F-protein showed ΔG= -14.22 kcal/mol and it was more stable than Q0. Additional, MTT and plate assays confirmed that virucidal Q1 effects occurs during adhesion step of cycle hRSV replication. In conclusion, acetylation improves anti-hRSV Quercetin effects because Quercetin pentaacetate could interact with F-protein with lower binding energy and better stability to block viral adhesion. These results show alternative anti-hRSV strategy and contribute to drug discovery and development.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionUniversidade Estadual Paulista UNESP (FCLAssis)-
Descrição: dc.descriptionUniversidade Estadual Paulista UNESP IBILCE-
Descrição: dc.descriptionCentro Multiusuário de Inovação Biomolecular (CMIB) Universidade Estadual Paulista UNESP IBILCE-
Descrição: dc.descriptionDepartment of Biology University of Rome Tor Vergata, Via della Ricerca Scientifica 1-
Descrição: dc.descriptionUniversidade Estadual Paulista UNESP (FCLAssis)-
Descrição: dc.descriptionUniversidade Estadual Paulista UNESP IBILCE-
Descrição: dc.descriptionCentro Multiusuário de Inovação Biomolecular (CMIB) Universidade Estadual Paulista UNESP IBILCE-
Descrição: dc.descriptionFAPESP: 2014/12298-7-
Idioma: dc.languageen-
Relação: dc.relationVirus Research-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAntiviral-
Palavras-chave: dc.subjectFlavonoids-
Palavras-chave: dc.subjectHEp-2 cells-
Palavras-chave: dc.subjectIn silico-
Palavras-chave: dc.subjectIn vitro-
Palavras-chave: dc.subjectRespiratory virus-
Título: dc.titleQuercetin pentaacetate inhibits in vitro human respiratory syncytial virus adhesion-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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