Design of a red-emitter hybrid material for bioimaging: europium complexes grafted on silica particles

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorMutti, A. M.G. [UNESP]-
Autor(es): dc.creatorSantos, J. A.O. [UNESP]-
Autor(es): dc.creatorCavalcante, D. G.S.M. [UNESP]-
Autor(es): dc.creatorGomes, A. S. [UNESP]-
Autor(es): dc.creatorJob, A. E. [UNESP]-
Autor(es): dc.creatorTeixeira, G. R. [UNESP]-
Autor(es): dc.creatorPires, A. M. [UNESP]-
Autor(es): dc.creatorLima, S. A.M. [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:23:50Z-
Data de disponibilização: dc.date.available2022-02-22T00:23:50Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2019-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.mtchem.2019.100204-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/198182-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/198182-
Descrição: dc.descriptionA bottom-up approach was used to design a high luminescent biomarker composed of nanoparticles of silica grafted with Eu3+ complex, and the labeling of CHO-k1 ovary cells and gastrocnemius muscle cells was successfully observed in imaging tests. Spheroidal silica nanoparticles were prepared via the solgel method and then decorated with Eu3+ complexes in a sequence of reactions to form the final hybrid nanoparticles. The material was characterized by scanning electron microscopy (120 ± 19 nm), zeta potential (−15 mV), and photoluminescence (red emission with 32% quantum efficiency). The material exhibit high biocompatibility with CHO-k1 cells, as evidenced via the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) method. CHO-k1 cells cultivated in the presence of the hybrid were investigated by microscopy techniques, and an active mechanism of internalization via phagocytosis was evidenced. Fluorescence and confocal images indicated red emission distributed in the cytoplasm, and especially around the nuclei. While for the muscle cells also stained with the luminescent particles, a distribution near the membrane was observed. Thus, the designed system proved to be safe and appropriate for use in bioimaging assays.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionSão Paulo State University (Unesp) School of Technology and Sciences-
Descrição: dc.descriptionSão Paulo State University (Unesp) Institute of Biosciences Humanities and Exact Sciences-
Descrição: dc.descriptionSão Paulo State University (Unesp) School of Technology and Sciences-
Descrição: dc.descriptionSão Paulo State University (Unesp) Institute of Biosciences Humanities and Exact Sciences-
Descrição: dc.descriptionFAPESP: 2018/13538–2-
Idioma: dc.languageen-
Relação: dc.relationMaterials Today Chemistry-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBiomarkers-
Palavras-chave: dc.subjectFunctionalization-
Palavras-chave: dc.subjectInorganic-organic hybrids-
Palavras-chave: dc.subjectLanthanides-
Palavras-chave: dc.subjectPhotoluminescence-
Título: dc.titleDesign of a red-emitter hybrid material for bioimaging: europium complexes grafted on silica particles-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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