Inflammatory response and macrophage polarization using different physicochemical biomaterials for oral and maxillofacial reconstruction

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorSagrado Coração University – USC-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorResearch and Education Center for Phototherapy in Health Science (Nupen)-
Autor(es): dc.creatorMunerato, Marcelo Salles-
Autor(es): dc.creatorBiguetti, Claudia Cristina [UNESP]-
Autor(es): dc.creatorParra da Silva, Raquel Barroso [UNESP]-
Autor(es): dc.creatorRodrigues da Silva, Ana Claudia [UNESP]-
Autor(es): dc.creatorZucon Bacelar, Ana Carolina [UNESP]-
Autor(es): dc.creatorLima da Silva, Jordan-
Autor(es): dc.creatorRondina Couto, Maira Cristina-
Autor(es): dc.creatorHúngaro Duarte, Marco Antônio-
Autor(es): dc.creatorSantiago-Junior, Joel Ferreira-
Autor(es): dc.creatorBossini, Paulo Sérgio-
Autor(es): dc.creatorMatsumoto, Mariza Akemi [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:23:33Z-
Data de disponibilização: dc.date.available2022-02-22T00:23:33Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-01-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.msec.2019.110229-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/198112-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/198112-
Descrição: dc.descriptionKnowledge about the action of immune system in the recognition of biomaterials has been extremely helpful when it comes about understanding host response and biomaterials' fate in human body. This study aimed to investigate inflammatory response and macrophage polarization during bone healing process of rat's calvaria critical defects using different bone materials in order to evaluate their influence on bone repair and on the quality of the newly formed bone tissue. Eighty male albinus Wistar rats underwent surgical procedure for the confectioning of a 5-mm diameter bone defect in their right parietal bone, and divided in four groups (n = 20 each), according the biomaterial: AG – Control, particulate intramembranous autogenous bone graft, HA/TCP – particulate biphasic calcium phosphate with HA/TCP (60/40), DBB – particulate deproteinized bovine bone, VC – particulate bioactive vitroceramic. After 3, 7, 21, and 45 days, the specimens were removed and prepared for microcomputed tomography (microCT), light and polarized microscopy, immunohistochemical analysis, and histomorphometry. No significant differences were detected considering percentage of leukocytes among the groups and periods, as well as in relation to immunolabeling for inflammatory (M1) and reparative (M2) macrophages. However, immunolabeling for bone marker indicated a delayed osteoblast differentiation in VC group, resulting in a decrease in mineralized bone matrix parameters in this group, revealed by microCT. In addition, AG and HA/TCP presented a satisfactory bone collagenous content. Despite the distinct origins and physicochemical properties of the tested biomaterials, they presented similar immune-inflammatory responses in the present experimental model, influencing bone-related proteins and bone quality, which must be considered according to their use.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Health Sciences Sagrado Coração University – USC, Rua Irmã Arminda 10-50-
Descrição: dc.descriptionDepartment of Basic Sciences São Paulo State University (Unesp) School of Dentistry, Rua José Bonifácio 1193-
Descrição: dc.descriptionDepartment of Dentistry Endodontics and Dental Materials Bauru School of Dentistry University of São Paulo – FOB/USP, Al. Octávio Pinheiro Brisola, 9-75-
Descrição: dc.descriptionResearch and Education Center for Phototherapy in Health Science (Nupen), Rua Pedro Fernandes Alonso, 766, Jardim Alvorada-
Descrição: dc.descriptionDepartment of Basic Sciences São Paulo State University (Unesp) School of Dentistry, Rua José Bonifácio 1193-
Descrição: dc.descriptionFAPESP: 03762-7-
Descrição: dc.descriptionFAPESP: 2016-
Idioma: dc.languageen-
Relação: dc.relationMaterials Science and Engineering C-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBiomaterials-
Palavras-chave: dc.subjectBone substitutes-
Palavras-chave: dc.subjectMacrophages-
Palavras-chave: dc.subjectOsteoimmunology-
Palavras-chave: dc.subjectRats-
Título: dc.titleInflammatory response and macrophage polarization using different physicochemical biomaterials for oral and maxillofacial reconstruction-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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