Exfoliative toxin E, a new Staphylococcus aureus virulence factor with host-specific activity

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Autor(es): dc.contributorTokyo University of Agriculture and Technology-
Autor(es): dc.contributorAgrocampus Ouest-
Autor(es): dc.contributorUniversidade Federal de Minas Gerais (UFMG)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade Federal da Bahia (UFBA)-
Autor(es): dc.creatorImanishi, Ichiro-
Autor(es): dc.creatorNicolas, Aurélie-
Autor(es): dc.creatorCaetano, Ana-Carolina Barbosa-
Autor(es): dc.creatorCastro, Thiago Luiz de Paula-
Autor(es): dc.creatorTartaglia, Natayme Rocha-
Autor(es): dc.creatorMariutti, Ricardo [UNESP]-
Autor(es): dc.creatorGuédon, Eric-
Autor(es): dc.creatorEven, Sergine-
Autor(es): dc.creatorBerkova, Nadia-
Autor(es): dc.creatorArni, Raghuvir K. [UNESP]-
Autor(es): dc.creatorSeyffert, Nubia-
Autor(es): dc.creatorAzevedo, Vasco-
Autor(es): dc.creatorNishifuji, Koji-
Autor(es): dc.creatorLe Loir, Yves-
Data de aceite: dc.date.accessioned2022-02-22T00:23:32Z-
Data de disponibilização: dc.date.available2022-02-22T00:23:32Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2019-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1038/s41598-019-52777-3-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/198109-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/198109-
Descrição: dc.descriptionExfoliative toxins (ETs) are secreted virulence factors produced by staphylococci. These serine proteases specifically cleave desmoglein 1 (Dsg1) in mammals and are key elements in staphylococcal skin infections. We recently identified a new et gene in S. aureus O46, a strain isolated from ovine mastitis. In the present study, we characterized the new et gene at a genetic level and the enzymatic activity of the deduced protein. The S. aureus O46 genome was re-assembled, annotated and compared with other publicly available S. aureus genomes. The deduced amino acid sequence of the new et gene shared 40%, 53% and 59% sequence identity to those of ETA, ETB and ETD, respectively. The new et gene shared the same genetic vicinity and was similar in other S. aureus strains bearing this gene. The recombinant enzyme of the new et gene caused skin exfoliation in vivo in neonatal mice. The new et-gene was thus named ete, encoding a new type (type E) of exfoliative toxin. We showed that ETE degraded the extracellular segments of Dsg1 in murine, ovine and caprine epidermis, as well as in ovine teat canal epithelia, but not that in bovine epidermis. We further showed that it directly hydrolyzed human and swine Dsg1 as well as murine Dsg1α and Dsg1β, but not canine Dsg1 or murine Dsg1γ. Molecular modeling revealed a correlation between the preferred orientation of ETE docking on its Dsg1 cleavage site and species-specific cleavage activity, suggesting that the docking step preceding cleavage accounts for the ETE species-specificity. This new virulence factor may contribute to the bacterial colonization on the stratified epithelia in certain ruminants with mastitis.-
Descrição: dc.descriptionLaboratory of Veterinary Internal Medicine Division of Animal Life Science Graduate School Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho-
Descrição: dc.descriptionSTLO INRA Agrocampus Ouest-
Descrição: dc.descriptionCellular and Molecular Genetics Laboratory Institute of Biological Sciences Federal University of Minas Gerais Belo Horizonte-
Descrição: dc.descriptionIBILCE/UNESP-
Descrição: dc.descriptionInstitute of Health Sciences Federal University of Bahia-
Descrição: dc.descriptionInstitute of Biology Federal University of Bahia-
Descrição: dc.descriptionIBILCE/UNESP-
Idioma: dc.languageen-
Relação: dc.relationScientific Reports-
???dc.source???: dc.sourceScopus-
Título: dc.titleExfoliative toxin E, a new Staphylococcus aureus virulence factor with host-specific activity-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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