Mycelium-bound lipase from Penicillium citrinum as biocatalyst for the hydrolysis of vegetable oils

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorScience and Technology Institute-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorLima, Rafaela T.-
Autor(es): dc.creatorAlves, Alex M.-
Autor(es): dc.creatorde Paula, Ariela V. [UNESP]-
Autor(es): dc.creatorde Castro, Heizir F.-
Autor(es): dc.creatorAndrade, Grazielle S.S.-
Data de aceite: dc.date.accessioned2022-02-22T00:23:28Z-
Data de disponibilização: dc.date.available2022-02-22T00:23:28Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2019-10-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.bcab.2019.101410-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/198080-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/198080-
Descrição: dc.descriptionMycelium-bound lipase from filamentous fungus has been extensively studied as an alternative biocatalyst used in biotransformation processes. In this work, Penicillium citrinum URM 4216 was assessed as a potential producer of mycelium-bound lipase and its catalytic activity was investigated to yield concentrated polyunsaturated fatty acids from vegetable oils. Under the established growth conditions and using olive oil as an inducer, P. citrinum was able to produce lipase having high mycelium-bound activity (271.67 ± 10.47 U g−1) revealing suitable biochemical (optimum pH = 8.0 at 45 °C) and kinetic (Km = 136.51 μmol L−1min−1, Vmax = 267.33 μmol g−1min−1) properties and thermal stability (half-life time of 1.8 h at 60 °C) to mediate biotransformation reactions. By applying factorial design, the hydrolysis of soybean oil yielded 38% at 38 °C using oil/buffer ratio of 20% in the presence of 2.5% of an emulsifying agent for 3 h. The hydrolysis degree was increased to 96% by replacing the conventional heating system for ultrasonic irradiation and increasing the incubation time to 9 h. Similar degrees of hydrolysis (>84%) were achieved using other rich polyunsaturated vegetable oils (sunflower, olive and canola oils); confirming the specificity of this mycelium-bound lipase for polyunsaturated fatty acids, such as oleic and linoleic acids.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)-
Descrição: dc.descriptionFederal University of Alfenas (UNIFAL-MG) Science and Technology Institute-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Pharmaceutical Sciences-
Descrição: dc.descriptionUniversity of São Paulo (USP) Engineering School of Lorena-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Pharmaceutical Sciences-
Descrição: dc.descriptionCAPES: 001-
Descrição: dc.descriptionFAPEMIG: APQ01391-18-
Idioma: dc.languageen-
Relação: dc.relationBiocatalysis and Agricultural Biotechnology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectHydrolysis-
Palavras-chave: dc.subjectMycelium-bound lipase-
Palavras-chave: dc.subjectPenicillium citrinum-
Palavras-chave: dc.subjectUltrasonic irradiation-
Palavras-chave: dc.subjectVegetable oils-
Palavras-chave: dc.subjectWhole-cells-
Título: dc.titleMycelium-bound lipase from Penicillium citrinum as biocatalyst for the hydrolysis of vegetable oils-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.