MicroRNA-mRNA Co-sequencing Identifies Transcriptional and Post-transcriptional Regulatory Networks Underlying Muscle Wasting in Cancer Cachexia

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniv Antioquia-
Autor(es): dc.contributorUniv Ghent-
Autor(es): dc.contributorUniv Southern Denmark-
Autor(es): dc.creatorFernandez, Geysson Javier [UNESP]-
Autor(es): dc.creatorFerreira, Juarez Henrique [UNESP]-
Autor(es): dc.creatorVechetti Jr, Ivan Jose [UNESP]-
Autor(es): dc.creatorMoraes, Leonardo Nazario de [UNESP]-
Autor(es): dc.creatorCury, Sarah Santiloni [UNESP]-
Autor(es): dc.creatorFreire, Paula Paccielli [UNESP]-
Autor(es): dc.creatorGutierrez, Jayson-
Autor(es): dc.creatorFerretti, Renato [UNESP]-
Autor(es): dc.creatorDal-Pai-Silva, Maeli [UNESP]-
Autor(es): dc.creatorRogatto, Silvia Regina-
Autor(es): dc.creatorCarvalho, Robson Francisco [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:21:26Z-
Data de disponibilização: dc.date.available2022-02-22T00:21:26Z-
Data de envio: dc.date.issued2020-12-10-
Data de envio: dc.date.issued2020-12-10-
Data de envio: dc.date.issued2020-05-29-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3389/fgene.2020.00541-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/197823-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/197823-
Descrição: dc.descriptionCancer cachexia is a metabolic syndrome with alterations in gene regulatory networks that consequently lead to skeletal muscle wasting. Integrating microRNAs-mRNAs omics profiles offers an opportunity to understand transcriptional and post-transcriptional regulatory networks underlying muscle wasting. Here, we used RNA sequencing to simultaneously integrate and explore microRNAs and mRNAs expression profiles in the tibialis anterior (TA) muscles of the Lewis Lung Carcinoma (LLC) model of cancer cachexia. We found 1,008 mRNAs and 18 microRNAs differentially expressed in cachectic mice compared with controls. Although our transcriptomic analysis demonstrated a high heterogeneity in mRNA profiles of cachectic mice, we identified a reduced number of differentially expressed genes that were uniformly regulated within cachectic muscles. This set of uniformly regulated genes is associated with the extracellular matrix (ECM), proteolysis, and inflammatory response. We also used transcriptomic data to perform enrichment analysis of transcriptional factor binding sites in promoter sequences, which revealed activation of the atrophy-related transcription factors NF-kappa B, Stat3, AP-1, and FoxO. Furthermore, the integration of mRNA and microRNA expression profiles identified post-transcriptional regulation by microRNAs of genes involved in ECM organization, cell migration, transcription factors binding, ion transport, and the FoxO signaling pathway. Our integrative analysis of microRNA-mRNA co-profiles comprehensively characterized regulatory relationships of molecular pathways and revealed microRNAs targeting ECM-associated genes in cancer cachexia.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionSao Paulo State Univ, Inst Biosci, Dept Struct & Funct Biol, Botucatu, SP, Brazil-
Descrição: dc.descriptionUniv Antioquia, Fac Med, Medellin, Colombia-
Descrição: dc.descriptionUniv Ghent, Dept Plant Biotechnol & Bioinformat, Ghent, Belgium-
Descrição: dc.descriptionUniv Southern Denmark, Dept Clin Genet, Univ Hosp Southern Denmark, Inst Reg Hlth Res, Odense, Denmark-
Descrição: dc.descriptionSao Paulo State Univ, Inst Biosci, Dept Struct & Funct Biol, Botucatu, SP, Brazil-
Descrição: dc.descriptionFAPESP: 12/13961-6-
Descrição: dc.descriptionFAPESP: 13/50343-1-
Descrição: dc.descriptionCNPq: 311530/2019-2-
Descrição: dc.descriptionFAPESP: 16/08294-1-
Descrição: dc.descriptionFAPESP: 14/13941-0-
Descrição: dc.descriptionFAPESP: 13/02005-0-
Descrição: dc.descriptionFAPESP: 11/16282-0-
Formato: dc.format16-
Idioma: dc.languageen-
Publicador: dc.publisherFrontiers Media Sa-
Relação: dc.relationFrontiers In Genetics-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectLewis Lung Cancer-
Palavras-chave: dc.subjectmiRNAs-
Palavras-chave: dc.subjecttranscription factors-
Palavras-chave: dc.subjectextracellular matrix-
Palavras-chave: dc.subjectcancer cachexia-
Título: dc.titleMicroRNA-mRNA Co-sequencing Identifies Transcriptional and Post-transcriptional Regulatory Networks Underlying Muscle Wasting in Cancer Cachexia-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.