Canine parvovirus 2b in fecal samples of asymptomatic free-living South American coatis (Nasua nasua, Linnaeus, 1766)

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Autor(es): dc.contributorUniversidade Estadual de Londrina (UEL)-
Autor(es): dc.contributorUniv Pitagoras Unopar-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorSpera, Caroline Giuseppa-
Autor(es): dc.creatorLorenzetti, Elis-
Autor(es): dc.creatorPereira Lavorente, Fernanda Louise-
Autor(es): dc.creatorMarques, Gustavo de Calasans [UNESP]-
Autor(es): dc.creatorBisca, Jacqueline Muniz [UNESP]-
Autor(es): dc.creatorTeixeira, Carlos Roberto [UNESP]-
Autor(es): dc.creatorAlfieri, Amauri Alcindo-
Autor(es): dc.creatorAlfieri, Alice Fernandes-
Data de aceite: dc.date.accessioned2022-02-22T00:20:08Z-
Data de disponibilização: dc.date.available2022-02-22T00:20:08Z-
Data de envio: dc.date.issued2020-12-10-
Data de envio: dc.date.issued2020-12-10-
Data de envio: dc.date.issued2020-05-14-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s42770-020-00293-2-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/197773-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/197773-
Descrição: dc.descriptionCanine parvovirus type 2 (CPV-2) is classified into three subtypes (CPV-2a, CPV-2b, and CPV-2c) and is the main cause of enteritis and myocarditis in young domestic and wild animals. This study aimed to evaluate the presence of CPV-2 in the feces of asymptomatic free-living coatis from Garden Forest Reserve, Palmital city, SP, Brazil. Fecal samples from 21 coatis (both sexes, different ages, and different aspects of feces) were collected in August 2014 and March 2015. The nucleic acid extracted was submitted to a polymerase chain reaction (PCR) assay to amplify a fragment of the VP2 gene of CPV-2. Eight (38%) fecal samples were positive in the PCR assay and were confirmed by sequencing. The 7 nucleotide (nt) sequences analyzed showed 100% nt identity with the prototype strain of CPV-2b (CPV-39 strain). The analysis of the deduced amino acid (aa) sequence revealed the presence of the GAT codon (aa D-Asp) at position 426 of the VP2 viral protein (subtype 2b). This study describes for the first time the identification of CPV-2b in asymptomatic free-living coatis (Nasua nasua) and suggests that coatis are susceptible to Carnivore protoparvovirus 1 infection and are important as a reservoir and an asymptomatic carrier to other wild and domestic animal species.-
Descrição: dc.descriptionUniv Estadual Londrina, Dept Vet Prevent Med, Lab Anim Virol, Celso Garcia Cid Rd,PR455 Km 380,POB 10011, BR-86057970 Londrina, Parana, Brazil-
Descrição: dc.descriptionUniv Estadual Londrina, Dept Vet Prevent Med, Mol Biol Unit, Multiuser Anim Hlth Lab, Celso Garcia Cid Rd,PR455 Km 380,POB 10011, BR-86057970 Londrina, Parana, Brazil-
Descrição: dc.descriptionUniv Pitagoras Unopar, Post Grad Program Anim Hlth & Prod, Arapongas, Parana, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista, Vet Hosp, Ctr Med & Res Wild Anim, POB 560, BR-18618000 Botucatu, SP, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista, Vet Hosp, Ctr Med & Res Wild Anim, POB 560, BR-18618000 Botucatu, SP, Brazil-
Formato: dc.format1399-1403-
Idioma: dc.languageen-
Publicador: dc.publisherSpringer-
Relação: dc.relationBrazilian Journal Of Microbiology-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectCoati-
Palavras-chave: dc.subjectFeces-
Palavras-chave: dc.subjectInfectious disease-
Palavras-chave: dc.subjectMolecular detection-
Palavras-chave: dc.subjectCPV-2b-
Título: dc.titleCanine parvovirus 2b in fecal samples of asymptomatic free-living South American coatis (Nasua nasua, Linnaeus, 1766)-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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